Dynamic expression pattern of Neuronal leucine-rich repeat 4 in mouse sensory neurons during development

小鼠感觉神经元发育过程中神经元富含亮氨酸重复序列4的动态表达模式

• 批准号: 20790164
• 负责人: BANDO Takayoshi
• 金额: $ 2.75万
• 依托单位: Wakayama Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20790164/
• 关键词: 細胞機能形態学; NLRR4; 脊髄神経節; シナプス形成; 接着因子; 一次感覚系; 神経発生

In this study, we examined the expression of NLRR-4 in the adult and developing dorsal root ganglia (DRGs). Northern blotting analysis revealed that the expression of NLRR-4 mRNA was detected between postnatal day (P) 0 and adult DRGs, in particular, the strong expression was observed at P0 and P7. In situ hybridization histochemistry showed that NLRR-4 mRNA was exclusively detected in the small-sized DRG neurons of the adult mice. Likewise, LacZ staining in NLRR-4 heterozygote mice revealed that LacZ-positive neurons were observed in 8% of the total DRG neurons, and this frequency was similar to the results of in situ hybridization histochemistry. Approximately 42% of NLRR-4-positive neurons contained receptor tyrosine kinase (Trk) A immunoreactivity, and 58% expressed Ret immunoreactivity in the adult mice. In the developing DRGs, NLRR-4 expression was first detected in 7% of total DRG neurons at embryonic day (E) 13.5, gradually increased, and reached the maximum level to 44% at E17.5. The percentage of LacZ-positive neurons in total neurons changed little between E17.5 and P7, and drastically reduced after P7. As a results of characterization for NLRR4-expressing neurons during development, NLRR4-positive neurons mainly expressed TrkC at E13.5. Between E17.5 and P0, NLRR-4-positive neurons contained predominantly TrkA-immunoreactivities. TrkC-positive neurons are earlier maturated compared with TrkA-positive neurons such as axonal innervation into the spinal cord, synaptic formation, and synaptic maturation. These results suggested that NLRR-4 might play a role in the synapse formation of DRG neurons contacting to the secondary neurons in the spinal cord.

在这项研究中，我们检查了成人和发展背根神经节（DRG）中NLRR-4的表达。 Northern印迹分析表明，在产后（P）0和成年DRG之间检测到NLRR-4 mRNA的表达，特别是在P0和P7处观察到强表达。原位杂交组织化学表明，在成年小鼠的小型DRG神经元中仅检测到NLRR-4 mRNA。同样，NLRR-4杂合子小鼠中的LACZ染色表明，在8％的总DRG神经元中观察到LacZ阳性神经元，并且该频率与原位杂交组织化学的结果相似。 NLRR-4阳性神经元中约有42％含有受体酪氨酸激酶（TRK）的免疫反应性，而成年小鼠中有58％表达了RET免疫反应性。在发展中的DRG中，NLRR-4表达在胚胎日（E）13.5的总DRG神经元的7％中首先被检测到，并逐渐增加，并达到E17.5时的最大水平至44％。总神经元中LACZ阳性神经元的百分比在E17.5和P7之间的变化很小，P7后大幅降低。作为在发育过程中表达NLR4的神经元表征的结果，NLRR4阳性神经元主要在E13.5处表达TRKC。在E17.5和P0之间，NLRR-4阳性神经元主要包含TRKA-免疫反应性。与TRKA阳性神经元（如轴突神经）相比，TRKC阳性神经元早期成熟。这些结果表明，NLRR-4可能在接触到脊髓中二级神经元的DRG神经元的突触形成中起作用。

项目成果

一次感覚神経の発生過程におけるNeuronal leucine-rich repeat 4の発現

初级感觉神经发育过程中神经元富含亮氨酸重复序列4的表达

• 发表时间: 2009
• 作者: 板東高功;森川吉博;宮島篤;仙波恵美子
• 通讯作者: 仙波恵美子