Analysis of molecular mechanism and cell-cell interactions in the establishment of left-right asymmetry

左右不对称建立的分子机制和细胞间相互作用分析

• 批准号: 11680714
• 负责人: TAMURA Koji
• 金额: $ 2.37万
• 依托单位: TOHOKU University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11680714/
• 关键词: Left-right asymmetry; retinoic acid; Pitx2; morphogenesis; flounder; 左右軸

Left-right asymmetry in heart and intestinal organs of vertebrates is produced in the process of their development. We investigated that shh, Pitx2 and retinoic acid are involved in this process, and that they are responsible for the left handedness. In particular, we cloned Pitx2 gene from zebrafish and analyzed its expression pattern, function, gene regulations, and interaction with retinoic acid (Tsukui et al., 1999).On the other hand, we cloned several members of T-box gene family, which are largely involved in organ for mation and patterning, in order to examine their functions on left-right asymmetry. We analyzed their expression patterns in zebrafish embryo (Yonei-Tamura et al., 1999 ; Tamura et al., 1999), and confirmed an important role of Tbx5/4 in chick embryo (Rodriguez-Esteban et al., 1999).We also started a project on flounder and left-right asymmetry. We examined and evaluated the visual chiasma as a significant phenotype of left-right asymmetry, and used as a guidepost of left-right asymmetry. We also cloned several PCR fragments of key genes on left-right asymmetry, includeing Pitx2, fgf8, Noda11, and msx (2) from the flounder.The above results were published in a review article (Tamura et al., 1999).

脊椎动物心脏和肠道器官的左右不对称是在发育过程中产生的。我们研究了 shh、Pitx2 和视黄酸参与了这个过程，并且它们与左利手有关。特别是，我们从斑马鱼中克隆了Pitx2基因，并分析了其表达模式、功能、基因调控以及与视黄酸的相互作用(Tsukui et al., 1999)。另一方面，我们克隆了T-box基因家族的几个成员，它们主要参与器官的形成和模式化，以检查它们在左右不对称性上的功能。我们分析了它们在斑马鱼胚胎中的表达模式（Yonei-Tamura 等，1999；Tamura 等，1999），并证实了 Tbx5/4 在鸡胚胎中的重要作用（Rodriguez-Esteban 等，1999）。还启动了一个关于比目鱼和左右不对称的项目。我们检查和评估了视觉交叉作为左右不对称的显着表型，并用作左右不对称的路标。我们还克隆了几个左右不对称关键基因的 PCR 片段，包括来自比目鱼的 Pitx2、fgf8、Noda11 和 msx (2)。上述结果发表在一篇综述文章中 (Tamura et al., 1999)。

项目成果

Tamura, K., Yonei-Tamura, S., and Izpisua Belmonte, J.C.: "Molecular basis of left-right asymmetry."Development, Growth & Differentiation. 61. 645-656 (1999)

Tamura, K.、Yonei-Tamura, S. 和 Izpisua Belmonte, J.C.：“左右不对称的分子基础。”发育、生长

Tamura,K.,Yonei Tamura,S.and Izpisua Belmonte,J.C.: "Molecular basis of left-right asymmetry"Development, Growth & Differentiation. 61. 645-656 (1999)

Tamura,K.、Yonei Tamura,S. 和 Izpisua Belmonte,J.C.：“左右不对称的分子基础”的发展、成长

Yonei-Tamura,S.,Tamura,K.,Tsukui,T.,and Belmonte,J.C.: "Spatially and temporal-restricted expression of two T-box genes during zebrafish embryogenesis."Mechanism of Development. 80. 219-221 (1999)

Yonei-Tamura,S.、Tamura,K.、Tsukui,T. 和 Belmonte,J.C.：“斑马鱼胚胎发生过程中两个 T 盒基因的空间和时间限制性表达。”发育机制。

Tsukui,T.,Capdevila,J.,Tamura K.et al.: "Multiple left-right asymmetry defects in Shh-/-mutant mice unveil a convergence of the Shh and Retinoic Acid pathways in the control of Lefty-1"Proc. Natl. Acad. Sci.. 96. 11376-11381 (1999)

Tsukui,T.、Capdevila,J.、Tamura K.等人：“Shh-/-突变小鼠中的多个左右不对称缺陷揭示了控制 Lefty-1 的 Shh 和视黄酸途径的收敛”Proc

Rodriguez-Esteban,C., et al.: "The T-box genes Tbx4 and Tbx5 regulate limb outgrowth and identity."Nature. 398. 814-818 (1999)

Rodriguez-Esteban, C. 等人：“T 盒基因 Tbx4 和 Tbx5 调节肢体的生长和身份。”《自然》。