Analysis of the regulatory mechanism by PRAT4A against TLR response

PRAT4A对TLR反应的调控机制分析

• 批准号: 20590487
• 负责人: TAKAMURA Sachiko
• 金额: $ 3.08万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590487/
• 关键词: TLR; PRAT4A; LPS; エンドトキシンショック

(1) The TLR4 region responsible for strong interaction with PRAT4A is very close to the site necessary for interaction with MD-2.(2) PRAT4A differentially interacts with each TLR and suggests that a single-nucleotide change in the PRAT4A gene influences not only the strength of TLR responses but can also alter the relative activity of each TLR.(3) Intracellular TLR4/MD-2 is responsible for unique set of LPS responses.

(1) 负责与 PRAT4A 强相互作用的 TLR4 区域非常接近与 MD-2 相互作用所需的位点。(2) PRAT4A 与每个 TLR 的相互作用存在差异，表明 PRAT4A 基因中的单核苷酸变化不仅影响TLR 反应的强度，但也可以改变每个 TLR 的相对活性。(3) 细胞内 TLR4/MD-2 负责一组独特的 LPS 反应。

项目成果

A Notch ligand, Delta-like 1 functions as an adhesion molecule.

Notch 配体 Delta-like 1 充当粘附分子。

• 发表时间: 2009
• 作者: Murata;A.;Okuyama;K.;Wagatsuma;K.;Moriyama;T.;Sakano;S.;Yagita;H.;Miyake;K.;Akashi-Takamura;S.;Yoshino;M.;Hayashi S.I.
• 通讯作者: Hayashi S.I.

Visualization of the molecular dynamics of lipopolysaccharide on the plasma membrane of murine macrophages by total internal reflection fluorescence microscopy.

通过全内反射荧光显微镜观察小鼠巨噬细胞质膜上脂多糖的分子动力学。

• 发表时间: 2008
• 期刊: J Biol Chem. 283巻
• 作者: Shawkat S;Karima R;Tojo T;Tadakuma H;Saitoh S;Akashi-Takamura S;Miyake K;Funatsu T;Matsushima K
• 通讯作者: Matsushima K

Detection of soluble PRAT4A (PRotein Associated with Toll-like receptor 4 ) by novel anti-PRAT4A monoclonal antibody.

通过新型抗 PRAT4A 单克隆抗体检测可溶性 PRAT4A（与 Toll 样受体 4 相关的蛋白质）。

• 发表时间: 2008
• 作者: Shibata Takuma;Takamura Sachiko;Richard Jennings;Miyake Kensuke
• 通讯作者: Miyake Kensuke

Soluble MD-1 detection using novel monoclonal antibodies.

使用新型单克隆抗体进行可溶性 MD-1 检测。

• 发表时间: 2008
• 作者: Jennings Richard;Akashi-Takamura Sachiko;Takuma Shibata;Toshihiko Kobayashi;Miyake Kensuke.
• 通讯作者: Miyake Kensuke.

Detection of soluble PRAT4A by novel anti-PRAT4A monoclonal antibody.

通过新型抗 PRAT4A 单克隆抗体检测可溶性 PRAT4A。

• 发表时间: 2008
• 作者: Hirahara K.;Yamashita M.;Iwamura C.;Shinoda K.;Hasegawa A.;Yoshizawa H.;Koseki H.;Gejyo F.;Nakayama T.;Takuma Shibata
• 通讯作者: Takuma Shibata