Local regulatory mechanisms of gastroduodenal bicarbonate secretion :

胃十二指肠碳酸氢盐分泌的局部调节机制：

• 批准号: 20590264
• 负责人: TAKEUCH Koji
• 金额: $ 3.08万
• 依托单位: Kyoto Pharmaceutical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590264/
• 关键词: 十二指腸アルカリ分泌; ガス状メディエーター; 硫化水素(H_2S); 一酸化炭素(CO); コリン作動薬(カルバコール); ムスカリン受容体サブタイプ; M受容体ノックアウトマウト; D細胞; ソマトスタチン; M受容体ノックアウトマウス; D細胞/ソマトスタチン; カルバコール; ムスカリン(M)受容体サブタイプ; 胃・十二指腸アルカリ分泌; 内因性メディエーター; ホスホジエステラーゼ; 輸送担体; 炭酸水; 炭酸脱水酵素

We demonstrated in the present study that 1) endogenous PGs and gas mediators such as NO, H_2S and CO, are involved in the local regulatory mechanism of the acid-induced duodenal HCO_3^- secretion, 2) NO and CO stimulate HCO_3^- secretion at least partly by increasing PG production, 3) the stimulatory action of H_2S is partly mediated by PG and NO as well as by capsaicin-sensitive afferent neurons, 4) PGE_2 stimulates HCO_3^- secretion via activation of EP3/EP4 receptors, and 5) both PDE1 and PDE3 are involved in the regulation of duodenal HCO_3^- secretion. In addition, we also found, using muscarinic (M) receptor knockout mice, that 6) the cholinergic stimulation of duodenal HCO_3^- secretion is mainly mediated by the activation of M1 and M3 receptors and modified by M4 receptors and that 7) the activation of M4 receptors inhibits the release of somatostatin from D cells and results in enhancement of the HCO_3^- response by removing the negative effect of somatostatin.

我们在本研究中证明1）1）内源性PG和诸如NO，H_2S和CO之类的内源性PGS和GAS介质参与了酸引起的Duodenal HCO_3^ - 分泌的局部调节机制，2）NO和CO刺激HCO_3^^至少部分通过pg的刺激性及其刺激性的作用，而不是pg的刺激性，而不是H_2的刺激作用，而不是h_2的刺激性。辣椒素敏感的传入神经元，4）PGE_2刺激HCO_3^ - 通过激活EP3/EP4受体分泌，5）PDE1和PDE3都参与了Duodenal HCO_3^的调节。 In addition, we also found, using muscarinic (M) receptor knockout mice, that 6) the cholinergic stimulation of duodenal HCO_3^- secretion is mainly mediated by the activation of M1 and M3 receptors and modified by M4 receptors and that 7) the activation of M4 receptors inhibits the release of somatostatin from D cells and results in enhancement of the HCO_3^- response by removing the生长抑素的负面影响。

项目成果

Muscarinic acetylcholine receptor subtypes involved in carbachol-induced HCO_3^- secretion in mouse duodenum in Vitro.

毒蕈碱乙酰胆碱受体亚型参与体外小鼠十二指肠中卡巴胆碱诱导的 HCO_3^- 分泌。

• 发表时间: 2010
• 作者: Takeuchi;et al
• 通讯作者: et al

EP1 receptor is involved in lubiprostone-stimulated HCO_3^- secretion in mouse stomach in vitro.

EP1 受体参与体外小鼠胃中鲁比前列酮刺激的 HCO_3^- 分泌。

• 发表时间: 2009
• 作者: Takahashi;et al.
• 通讯作者: et al.

胃アルカリ分泌に対する硫化水素の影響

硫化氢对胃碱性分泌的影响

• 发表时间: 2008
• 期刊: Ulcer Research 35
• 作者: 伊勢文孝;他
• 通讯作者: 他

Lubiprostone stimulates HCO_3^- secretion in isolated mouse stomachs in vitro through activation of prostaglandin EP1 receptors.

Lubiprostone 通过激活前列腺素 EP1 受体，在体外刺激离体小鼠胃中 HCO_3^- 的分泌。

• 发表时间: 2010
• 作者: Masafumi K;Shusaku H;Kento T;Koji T
• 通讯作者: Koji T

Role of calcium-sensing receptor (CaSR) on gastric acidsecretion in isolated mouse stomachs.

钙敏感受体（CaSR）对离体小鼠胃胃酸分泌的作用。

• 发表时间: 2010
• 作者: Masafumi K、Shusaku H;Eitaro A;Shinichi K;Koji T
• 通讯作者: Koji T