Analysis of functional significance of lipids in receptors for vision, hearing and olfaction by way of functional analysis of fatty acid binding proteins

通过脂肪酸结合蛋白的功能分析来分析视觉、听觉和嗅觉受体中脂质的功能意义

基本信息

批准号：
20590180
负责人：
SAINO Sachiko
金额：
$ 3万
依托单位：
Tohoku Bunka Gakuen University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2008
资助国家：
日本
起止时间：
2008 至 2010
项目状态：
已结题

项目摘要

In the retina of normal adult mice, B-FABP was mainly localized in the cone photoreceptor cells, H-FABP in some populations of amacrine/bipolar/horizontal interneurons, and E-FABP in ganglion cells, suggesting the discrete functional involvement of FABPs and their ligands, FAs in the photoreception and photo-transmission. In addition, A-FABP-like immunoreactivity was located in resident microglia of normal retinae. In damaged retinae following photic injury, E-FABP was intensely localized in invasive macrophages, allowing discrete identification of the resident microglia and invasive macrophages by A- and E-FABP immunoreactivity, respectively. In the inner ear of normal adult mice, H (heart- type) -FABP was localized in inner and outer pillar cells and outer phalangeal cells, while B (brain-type)-FABP was localized in border cells and cells of Hensen, and fibrocytes in the spiral limbus and spiral prominence. However, no hair cells expressed any species of FABPs. Althoug the FABP species and/or their ligands, FAs, is suggested to play important roles in the regulation of the hearing function, mutant mice with deletion of the gene for either H- or B-FABP did not show any impairment of the hearing ability. In addition to the two sensory organs, E-FABP was expressed/localized in most, if not all, populations of the dendritic cells in the subepithelial domes, follicles and interfollicular regions of Peyer's patches and presumptive macrophages in their germinal centers, and all M cells in the follicle-associated epithelium of mouse intestine. The immunoreactivity in both of the cell populations makes it easy to recognize the accumulation of DCs in the subepithelial domes in close proximity to the base of M cells, which is essential for luminal antigens to be transported to Peyer's patches. E-FABP may play some important roles in the mucosal immune reaction through Peyer's patches and associated structures.

在正常成年小鼠的视网膜中，B-FABP主要定位于锥形光感受器细胞中，H-FABP在某些无花蛋白/双极/双极/水平中间神经元中，以及在神经节细胞中的电子fabp，表明Fabps的离散功能涉及Fabps及其液体的功能涉及，fas及其液体及其光摄取和照片摄取和照片。另外，在正常视网膜的居民小胶质细胞中，A-FABP样免疫反应性。在光损伤后受损的视网膜损伤中，E-FABP在侵袭性巨噬细胞中被强烈定位，从而可以通过A和E-FABP免疫反应性离散地鉴定驻留小胶质细胞和侵入性巨噬细胞。在正常成年小鼠的内耳中，H（心脏型）-FABP位于内部和外柱细胞和外脊椎细胞中，而B（脑型）-FABP位于Hensen的边界细胞和细胞中，以及螺旋状的Limbus和螺旋出现的纤维细胞。但是，没有毛细胞表达任何种类的Fabps。 Altig fabp物种和/或其配体FAS被建议在听力功能的调节中起重要作用，对H-或B-FABP缺乏基因的突变小鼠没有显示出听力能力的任何损害。除了两个感觉器官外，在大多数（如果不是全部）（如果不是全部）中，E-FABP在上皮下圆顶，卵泡和peyer斑块的斑块间区域中的树突状细胞的种群以及其生长中心的假定巨噬细胞的所有细胞，以及所有M型细胞中的M型细胞，以及所有M型细胞中的M型细胞。两个细胞群体中的免疫反应性使得靠近M细胞底部的DC在上皮下圆顶中的积累变得容易，这对于将腔抗原运送到Peyer的斑块至关重要。 E-FABP可以通过Peyer的斑块和相关结构在粘膜免疫反应中起一些重要作用。