Monitoring of natural zearalenone contamination in feeds of cattle herds, with special focus on the effect on reproductive efficacy.

监测牛群饲料中的天然玉米赤霉烯酮污染，特别关注对繁殖功效的影响。

• 批准号: 20580355
• 负责人: TAKAGI Mitsuhiro
• 金额: $ 3.08万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20580355/
• 关键词: ウシ; ブタ; マイコトキシン; ゼアラレノン; 尿; 卵胞液; 尿サンブル; 卵胞液サンプル; マイコトシキン吸着剤; 尿サンプル; LC; MS

The aims of the first study were (1) to investigate the efficacy of measuring bovine urinary zearalenone (ZEN) concentrations by using a commercially available ELISA method in cattle kept under different feeding conditions to monitor the natural contamination of feeds at the farm level, and (2) to investigate the effects of supplementation of a mycotoxin adsorbent (MA) product in the feed based on urinary ZEN concentration. The findings indicate the usefulness of measuring concentrations of urinary ZEN and its metabolites not only for monitoring the natural ZEN contamination of cattle feed at the farm level but also for in vivo evaluation of MA function after supplementing feeds with MA.Zearalenone (ZEN) and its metabolites are important nonsteroidal estrogenic mycotoxins that cause reproductive disorders in domestic animals, especially pigs. The aim of the second study were to simultaneously detect ZEN and its metabolites α-zearalenol (α-ZOL) and β-zearalenol (β-ZOL) in porcine follic … More ular fluid (FF) by liquid chromatography-tandem mass spectrometry. ZEN and α-ZOL, but not β-ZOL, were detected in all pooled FF samples collected from coexisting follicles (diameter >6mm) within 10 ovaries. Furthermore, ZEN and α-ZOL were detected in samples pretreated with β-glucuronidase/arylsulfatase, but not in those left untreated, suggesting that the FF samples contained glucuronide-conjugated forms of the mycotoxins that may be less harmful to porcine oocytes due to glucuronidation affecting the receptor binding. Nonetheless, the effects of the glucuronide-conjugated forms should be studied, both in vitro and in vivo. Additionally, the influence of acute exposure to zearalenone (ZEN) on porcine oocyte maturation, fertilization, or sperm penetration ability during both in vitro maturation and fertilization was evaluated. First, oocytes were cultured in ZEN-containing (0-1000μg/l) maturation medium and then fertilized. Second, oocytes maturing in vitro without ZEN were fertilized in ZEN-containing fertilization medium. The maturation rates of oocytes and penetration ability of sperm decreased significantly in the presence of 1000μg/l of ZEN. However, neither increases in the rates of degeneration and DNA fragmentation of oocytes nor reductions in normal and polyspermic fertilization were observed. ZEN did not affect the sperm penetration rates, however, 1000μg/l ZEN had positive effects on normal and polyspermic fertilization rates. Therefore, it can be suggested that an acute exposure of porcine oocytes during maturation, and oocytes and sperm during fertilization to ZEN up to 1000μg/l may not affect the fertility of the oocytes. Less

第一项研究的目的是（1）研究通过在牛中使用商用可用的ELISA方法测量牛尿尿素酮（ZEN）浓度的有效性，以保持不同的饲料条件下，以监测农场饲料在农场水平上的自然污染，（2）研究基于饲料的mycotoxin Adsorbent（MA）浓度（MA）饲料（MA）的浓度（MA）。研究结果表明，测量尿ZEN及其代谢产物的测量浓度不仅用于监测农场水平上的牛饲料的自然Zen污染，而且还可以在补充Ma.Zearalenone（ZEN）及其代谢物及其代谢物及其代谢物及其重要的非雌激素的动物毒素的情况下对MA功能进行体内评估，这是尤其是毒素的毒素。第二项研究的目的是通过液态色谱量表质谱法同时检测Zen及其代谢产物α-成乙烯醇（α-Zol）和β-酶（β-Zol）（β-Zol）。在10个卵巢中从共存的卵泡（直径> 6mm）中收集的所有合并的FF样品中检测到ZEN和α-ZOL，而不是β-Zol。此外，在用β-葡萄糖醛酸酶/芳基硫糖酶预处理的样品中检测到Zen和α-Zol，但在未经处理的那些样品中未检测到，这表明FF样品中含有糖蛋白酶结合形式的mycotoxin形式，可能对替代型鸡蛋素造成的mycivation cocivation devirative turaniD compatiation turaniD compation deviration duraniD。尽管如此，糖苷缀合的形式的作用应在体外和体内进行研究。此外，评估了评估急性暴露于Zearalenone（ZEN）对猪卵母细胞成熟，受精或精子穿透能力期间在体外成熟和受精过程中的影响。首先，将卵母细胞培养在含禅宗（0-1000μg/L）成熟培养基中，然后受精。其次，在含Zen的受精培养基中，在没有ZEN的体外成熟卵母细胞。在存在1000μg/l Zen的情况下，卵母细胞的成熟速率和精子的穿透能力显着降低。然而，既没有观察到卵母细胞的变性和DNA片段化的速率，也没有观察到正常和多性经历受精的降低。 ZEN不影响精子渗透率，但是，1000μg/L Zen对正常和多体经体受精率有积极影响。因此，可以建议在成熟过程中急性暴露猪卵母细胞，在受精过程中，卵母细胞和精子却可能不会影响卵母细胞的生育能力。较少的

项目成果

Detection of zearalenone and Its metabolites in naturally contaminated follicular fluids by using LC/MS/MS and in vitro effects of zearalenone on oocvte maturation in cattle.

使用 LC/MS/MS 检测自然污染的卵泡液中的玉米赤霉烯酮及其代谢物以及玉米赤霉烯酮对牛卵母细胞成熟的体外影响。

• 发表时间: 2008
• 期刊: Reproductive Toxicology 26
• 作者: Takaei M.;et al.
• 通讯作者: et al.

牛尿中ゼアラレノン濃度を指標とした給与飼料中ゼアラレノン浸潤動態モニター法の確立

以牛尿中玉米赤霉烯酮浓度为指标监测饲料中玉米赤霉烯酮渗透动态的方法的建立

• 发表时间: 2010
• 作者: 志賀慧;高木光博;宇野誠一;蓮沼浩;岡本光司;出口栄三郎
• 通讯作者: 出口栄三郎

Detection of zearalenone and its metabolites in naturally contaminated follicular fluids by using LC/MS/MS and in vitro effects of zearalenone on oocyte maturation in cattle.

使用 LC/MS/MS 检测自然污染的卵泡液中的玉米赤霉烯酮及其代谢物，以及玉米赤霉烯酮对牛卵母细胞成熟的体外影响。

• 发表时间: 2008
• 期刊: Reprod Toxicol. 26
• 作者: Takagi M.;Mukai S.;Kuriyagawa T.;Takagaki K.;Uno S.;Kokushi E.;Otoi T.;Budiyanto A.;Shirasuna K.;Miyamoto A.;Kawamura O.;Okamoto K.;Deguchi E.
• 通讯作者: Deguchi E.

牛尿中ゼアラレノン濃度を指標とした給与飼料中ゼアラレノン浸潤動態モニター法の確率

以牛尿中玉米赤霉烯酮浓度为指标的饲料中玉米赤霉烯酮渗透动态监测方法的概率

• 发表时间: 2009
• 作者: 志賀慧;高木光博;宇野誠一;國師恵美子;蓮沼浩;松本大策;岡本光司;出口栄三郎
• 通讯作者: 出口栄三郎