Establishment of Detection Method of Endocrine Disrupter by Avian Yolk-Related Gene Expression

禽类卵黄相关基因表达检测内分泌干扰物方法的建立

• 批准号: 20580307
• 负责人: MORI Makoto
• 金额: $ 3.08万
• 依托单位: Shizuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20580307/
• 关键词: 代謝; 内分泌制御; 畜産学; 内分泌; 発生・分化; 家禽; 内分泌撹乱; ウズラ; ホルモン; 肝臓; 卵黄; 代謝; 内分泌制御; 畜産学; 内分泌; 発生・分化; 家禽; 内分泌撹乱; ウズラ; ホルモン; 肝臓; 卵黄

A sensitive method by using the gene expression related to avian yolk protein was established to detect the effect of endocrine disrupters. Especially focusing on oogenesis and embryonic growth, the most appropriate gene is suggested to be quail very-low-density-lipoprotein. The effect of heavy metals and antioxidants as well as diethylstilbestrol was examined with the causal relationship with dose-related manner at molecular level.

建立了使用与禽蛋白蛋白相关的基因表达的敏感方法来检测内分泌不顾问的作用。特别是专注于卵子发生和胚胎生长，建议最合适的基因是鹌鹑非常低密度脂蛋白。在分子水平上检查了重金属和抗氧化剂以及二乙基苯甲酸酯的影响。