Establishment of Detection Method of Endocrine Disrupter by Avian Yolk-Related Gene Expression

禽类卵黄相关基因表达检测内分泌干扰物方法的建立

• 批准号: 20580307
• 负责人: MORI Makoto
• 金额: $ 3.08万
• 依托单位: Shizuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20580307/
• 关键词: 代謝; 内分泌制御; 畜産学; 内分泌; 発生・分化; 家禽; 内分泌撹乱; ウズラ; ホルモン; 肝臓; 卵黄

A sensitive method by using the gene expression related to avian yolk protein was established to detect the effect of endocrine disrupters. Especially focusing on oogenesis and embryonic growth, the most appropriate gene is suggested to be quail very-low-density-lipoprotein. The effect of heavy metals and antioxidants as well as diethylstilbestrol was examined with the causal relationship with dose-related manner at molecular level.

建立了一种利用禽蛋黄蛋白相关基因表达检测内分泌干扰物效应的灵敏方法。特别关注卵子发生和胚胎生长，建议最合适的基因是鹌鹑极低密度脂蛋白。在分子水平上以剂量相关方式检查重金属和抗氧化剂以及己烯雌酚的影响。

项目成果

Effect of Selenium on Cellular Glutathione Peroxidase mRNA Expression and Consequent Hatchability in Japanese Quail (Coturnix japonica).

硒对日本鹌鹑 (Coturnix japonica) 细胞谷胱甘肽过氧化物酶 mRNA 表达及其孵化率的影响。

• 发表时间: 2008
• 作者: Sopon Wilaison;Makoto Mori
• 通讯作者: Makoto Mori

Effect on Selenium on Hatchability and Glutathione Peroxidase mRNA Expression during Embryogenesis in Japansese Quail.

硒对日本鹌鹑胚胎发生过程中孵化率和谷胱甘肽过氧化物酶 mRNA 表达的影响。

• 发表时间: 2009
• 作者: Sopon Wilaison;Makoto Mori
• 通讯作者: Makoto Mori

Cloning and Express ion of Cellular Glutathione Peroxidase (GPX1) in Japanese Quail (Coturnix japonica).

日本鹌鹑（Coturnix japonica）细胞谷胱甘肽过氧化物酶（GPX1）的克隆和表达。

• 发表时间: 2009
• 期刊: Journal of Poultry Science 46
• 作者: Sopon Wilaison;Makoto Mori
• 通讯作者: Makoto Mori

The Biological Monitoring of Wild Birds ; The Cadmium Content of Organs from Migratory Birds.

野生鸟类的生物监测；

• 发表时间: 2009
• 作者: Mariko Mochizuki;Makoto Mori;Hiroshi Kajigaya;Shin-ichi Hayama;Yoshitsugu Ochiai;Ryo Hondo;Fukiko Ueda
• 通讯作者: Fukiko Ueda

Impact, Monitoring and Management of Environmental Pollution(Chapter 15. A Cadmium Standard Regression Line : A Possible New Index for Biological Monitoring.)(Ed.A.El Numr)

环境污染的影响、监测和管理（第 15 章：镉标准回归线：一个可能的生物监测新指标。）（Ed.A.El Numr）

• 发表时间: 2010
• 作者: Mariko Mochizuki;Makoto Mori;Ryo Hondo;Fukiko Ueda
• 通讯作者: Fukiko Ueda