Comparative study on the toxic factors of Chochlodinium polykrikoides and Chattonella marina

多环藻与海藻的毒性因素比较研究

• 批准号: 20580204
• 负责人: ODA Tatsuya
• 金额: $ 3万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20580204/
• 关键词: プランクトン; 赤潮; 毒性; 活性酸素; 鞭毛藻類; 粘液

Generation of reactive oxygen species (ROS) by Chattonella marina could be confirmed by ESR spin trapping method. Immunohistochemical analysis of gill lamellae from yellowtail exposed to C.marina using antiserum against crude glycocalyx demonstrated that there were the antigns recognaised by the antiserum on the surface of gill lamellae. As compared to C.marina, the levels of ROS detected in Cochlodinium polykrikoides were trace levels. Furthermore, no significant increase in O^2_- generation by C.polykrikoides was observed in the presence of lectins or fish mucus prepared from skin and gill of yellowtail, whereas C.marina generated increased level of O^2_- responding to these stimuli. The cell-free aqueous extract prepared from C.polykrikoides showed toxic effect on HeLa cells, but the extract of C.marina had almost no effect. Furthermore, gradual accumulation of polysaccharides in the medium was observed during the culture of C.polykrikoides, but no such change was observed in the medium of C.marina. These results suggest that ROS may play a significant role in the fish-killing activity of C.marina, whereas in the case of C.polykrikoides, certain toxic substances or polysaccharides are mainly responsible for the toxicity rather than ROS.

Chattonella码头可以通过ESR旋转捕获方法确认活性氧（ROS）。使用抗血清暴露于c.marina的gill薄片的免疫组织化学分析使用抗血清暴露于c.marina，这表明有抗血清在吉尔薄片表面上被抗血清所包含的抗凝胶。与c.marina相比，在耳氯二酮中检测到的ROS水平是痕量水平。此外，在存在于凝集素或鱼粘液的存在下，c. polykrikoides的O^2_-生成没有显着增加，而c.marina对这些刺激的反应水平提高了。从丙氏二丙烯酸杆菌制备的无细胞水提取物对HeLa细胞显示出毒性作用，但c.marina提取物几乎没有作用。此外，观察到培养基培养培养基中多糖在培养基中的逐渐积累，但是在c.marina培养基中未观察到这种变化。这些结果表明，ROS可能在c.marina的杀鱼活性中起重要作用，而在C. polykrikoides的情况下，某些有毒物质或多糖主要是造成毒性而不是ROS的原因。

项目成果

Cytotoxic action mode of a novel porphyrin derivative isolated from harmful red tide dinoflagellate Heterocapsa circularisquama.

从有害赤潮甲藻 Heterocapsa circularisquama 中分离出的新型卟啉衍生物的细胞毒性作用模式。

• 发表时间: 2008
• 期刊: J.Biochem.Mol.Toxicol. 22
• 作者: D.Kim;Y.Miyazaki;T.Nakashima;T.Iwashita;T.Fujita;K.Yamaguchi;K.-S.Choi;T.Oda
• 通讯作者: T.Oda

Comparison of the responses of peritoneal macrophages from Japanese flounder (Paralichthys olivaceus) against high-irulent and low-virulent strains of Edwardsiella tarda.

日本牙鲆（Paralichthys olivaceus）腹膜巨噬细胞对高毒力和低毒力爱德华氏菌菌株的反应比较。

• 发表时间: 2008
• 期刊: Fish and Shellfish Immunology 24
• 作者: K.Ishibe;K.Osatom;K.Hara;K.Kanai;K.Yamaguchi;T.Oda
• 通讯作者: T.Oda

未利用天然資源として有望な赤潮プランクトン

赤潮浮游生物是一种有前途的未开发自然资源

• 发表时间: 2009
• 期刊: 養殖 4
• 作者: Yagi Y;I Kinoshita;S Fujita;H Ueda;D Aoyama.;伊藤毅史・古満啓介・山口敦子;鈴木秀和;伊東優太;山口敦子・古満啓介・久米元;小田達也
• 通讯作者: 小田達也

Molecular mechanism of ROS (reactive oxygen species) generation by unicellular marine phytoplankton Chattonella marina.

单细胞海洋浮游植物查托氏菌码头产生ROS（活性氧）的分子机制。

• 发表时间: 2009
• 作者: D.Kim;T.Oda.
• 通讯作者: T.Oda.

Comparative analysis of the production of nitric oxide(NO)and tumor necrosis factor-a(TNF-a)from macrophages exposed to high virulent and low virulent strains of Edwardsiella tarda.

暴露于高毒力和低毒力爱德华氏菌菌株的巨噬细胞产生一氧化氮（NO）和肿瘤坏死因子-a（TNF-a）的比较分析。

• 发表时间: 2009
• 期刊: Fish & Shellfish Immunology, 27
• 作者: K.Ishibe;T.Yamanishi;Y.Wang;K.Osatomi;K.Hara;K.Kanai;K.Yamaguchi;T.Oda.
• 通讯作者: T.Oda.