Studies on the structure and function of the nucleus in senescent cells

衰老细胞细胞核结构与功能的研究

• 批准号: 20370079
• 负责人: ISHIKAWA Fuyuki
• 金额: $ 8.65万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20370079/
• 关键词: テロメア; 細胞老化; NF-kappaB; ヘテロクロマチン; ヒストンH1; HMGA; lamin; NFkappaB

We have previously reported that histone H1 disappears and HMGA proteins are increased in cellular senescent cells. In this study, we further examined the structure and function of the nucleus in senescent cells. We found that 1) histone H1 is likely degraded via the ubiqutin-proteasome pathway, 2) the NF-kB pathway is involved in inducing the characteristic gene expression profiles in senescent cells, and 3) the nuclear membrane is structurally and biochemically dysfunctional. These results will provide a means to control the senescence phenotypes.

我们先前已经报道了组蛋白H1消失，并且在细胞衰老细胞中HMGA蛋白增加。在这项研究中，我们进一步检查了衰老细胞中细胞核的结构和功能。我们发现1）组蛋白H1可能通过ubiqutin-------途径降解，2）NF-KB途径参与诱导衰老细胞中的特征基因表达谱，而3）核膜在结构和生物化学上是功能障碍的。这些结果将提供一种控制衰老表型的手段。

项目成果

Interaction between DNMTl and DNA replication reactions in the SV40 in vitro replication system.

SV40体外复制系统中DNMT1和DNA复制反应之间的相互作用。

• 发表时间: 2008
• 期刊: Cancer science 99
• 作者: Shimamura;S.
• 通讯作者: S.

RPA-like Mammalian Ctc1-Stn1-Ten1 Complex Binds to Single-Stranded DNA and Protects Telomeres Independently of the Pot1 Pathway

• DOI: 10.1016/j.molcel.2009.08.009
• 发表时间: 2009-10-23
• 期刊: MOLECULAR CELL
• 影响因子: 16
• 作者: Miyake, Yasuyuki;Nakamura, Mirai;Ishikawa, Fuyuki
• 通讯作者: Ishikawa, Fuyuki

Low-Dose Stress Responses

低剂量应激反应

• 发表时间: 2009
• 作者: Nakamura;M.;Nabetani;A.;Mizuno;T.;Hanaoka;F.;Ishikawa;F.;Fuyuki Ishikawa;Fuyuki Ishikawa;Fuyuki Ishikawa;石川冬木;石川冬木;石川冬木;石川冬木;Fuyuki Ishikawa;Fuyuki Ishikawa;Fuyuki Ishikawa;Fuyuki Ishikawa
• 通讯作者: Fuyuki Ishikawa

Unusual Telomeric DNAs in Human Telomerase-Negative Immortalized Cells

• DOI: 10.1128/mcb.00603-08
• 发表时间: 2009-02-01
• 期刊: MOLECULAR AND CELLULAR BIOLOGY
• 影响因子: 5.3
• 作者: Nabetani, Akira;Ishikawa, Fuyuki
• 通讯作者: Ishikawa, Fuyuki

Fission yeast Vps1 and Atg8 contribute to oxidative stress resistance

• DOI: 10.1111/j.1365-2443.2009.01376.x
• 发表时间: 2010-03-01
• 期刊: GENES TO CELLS
• 影响因子: 2.1
• 作者: Mikawa, Takumi;Kanoh, Junko;Ishikawa, Fuyuki
• 通讯作者: Ishikawa, Fuyuki