Structural analysis of genomic regions where genetic recombination was suppressed for over million years

对基因重组被抑制数百万年的基因组区域进行结构分析

• 批准号: 20310118
• 负责人: ABE Kuniya
• 金额: $ 12.4万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20310118/
• 关键词: ゲノム進化; 再編; 染色体組換え; t-ハプロタイプ; BACライブラリー; ゲノム再編成; ゲノム進化・再編

Goal of this study is to elucidate the structure and divergence of t-haplotype genome in which genetic recombination has been suppressed for over million years. Large scale DNA rearrangements including deletions and duplications were revealed by Array-CGH method, inferring the break points of inversions associated with t-haplotypes. BAC genomic library was constructed from tw5 haplotype, and clones corresponding to the t-complex region were isolated and sequenced. Exceptionally high incidence of SNPs (single nucleotide polymorphisms) has been detected between the tw5haplotype and the standard laboratory mouse strain.

这项研究的目的是阐明遗传重组已抑制了超过百万年的T-型型基因组的结构和差异。通过Array-CGH方法揭示了包括缺失和复制在内的大型DNA重排，从而推断了与T-Haplotypes相关的反转的断裂点。 BAC基因组文库是由TW5单倍型构建的，并分离了与T-复合区域相对应的克隆并测序。在TW5HAPLOTYPE和标准实验室小鼠菌株之间已经检测到SNP（单核苷酸多态性）的异常高（单核苷酸多态性）。

项目成果

New evidence confirms that the mitochondrial bottleneck is generated without reduction of mitochondrial DNA content in early primordial germ cells of mice.

• DOI: 10.1371/journal.pgen.1000756
• 发表时间: 2009-12
• 期刊: PLoS genetics
• 影响因子: 4.5
• 作者: Cao L;Shitara H;Sugimoto M;Hayashi J;Abe K;Yonekawa H
• 通讯作者: Yonekawa H

Highly parallel SNP genotyping reveals high-resolution landscape of mono-allelic Ube3a expression associated with locus-wide antisense transcription.

• DOI: 10.1093/nar/gkq1201
• 发表时间: 2011-04
• 期刊: Nucleic acids research
• 影响因子: 14.9
• 作者: Numata K;Kohama C;Abe K;Kiyosawa H
• 通讯作者: Kiyosawa H

マウス亜種間の兄妹交配で生じる雑種崩壊現象の表現型解析

小鼠亚种间兄妹交配引起的杂种崩溃的表型分析

• 发表时间: 2008
• 作者: 津田薫;持田慶司;吉木淳;森脇和郎;阿部訓也
• 通讯作者: 阿部訓也

Genetic variation of melatonin productivity in laboratory mice under domestication.

驯化条件下实验室小鼠褪黑激素生产力的遗传变异。

• 发表时间: 2010
• 期刊: Proc.Natl.Acad.Sci, USA 107
• 作者: Kasahara T.;Abe K.;Mekada K.;Yoshiki A.;Kato;T.
• 通讯作者: T.

Genetic Differences among C57BL/6 Substrains

• DOI: 10.1538/expanim.58.141
• 发表时间: 2009-04-01
• 期刊: EXPERIMENTAL ANIMALS
• 影响因子: 2.4
• 作者: Mekada, Kazuyuki;Abe, Kuniya;Yoshiki, Atsushi
• 通讯作者: Yoshiki, Atsushi