Development technique of bone regeneration using Mesenchymal Stem Cell

利用间充质干细胞进行骨再生的开发技术

• 批准号: 11307048
• 负责人: UEDA Minoru
• 金额: $ 24.47万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11307048/
• 关键词: Cultured Bone; Tissue Engineering; Marrow Cell; Carrier; Bone Regeneration; 組織再生; 培養

Recent studies in the field of tissue engineering have developed and established the technique of cultured bone regeneration with mesenchymal stem cells (MSCs) derived from bone marrow. Most of characteristics of the regenerat ion have been clarified by these recent studies. Generally, it have described a new technology called tissue engin eering that involves the morphogenesis of new tissue from constructs formed of isolated cells, biocompatible scaff olds and growth factors. In the present studies, we used MSCs as cell sources, ceramics and absorbable collagen as matrices, and rhBMP-2 and Platelet Rich Plasma as growth factors. In order to examine the effectiveness of cultur ed bone regeneration for clinical use, we investigated the following five things : 1) development of matrix, 2) ost eogenesis in vivo, 3) establishment of the method of larger scale of osteoblast culture, 4) the effectiveness of growth factors to improve the bone formation. 5) A possibility of clinical application in human. In regard to the matrix, we investigated the osteogenic potential of non resorbable HA and a new biodegradable β-tricalcium phosph ate (β-TCP), showing that β-TCP would be an ideal scaffolds for bone regeneration. In addition, we found that absorbable collagen would be an effective scaffold for rhBMP-2 in the sinus as well as autogenous bone. However, m ost studies regarding bone formation have adopted two dimensional histomorphometric evaluations and molecular biol ogys, not three dimensional such as micro CT. We also successfully evaluated microstructure of trabecular bone in human clinical cases. In some years from now, we are going to perform studies to improve the utility of the graft for the periodontal or alveolar defect for clinical use of technique of the cultured bone regeneration using MSCs with plasticity.

组织工程领域的最新研究已经开发并建立了利用源自骨髓的间充质干细胞（MSC）进行培养骨再生的技术。一般来说，这些最近的研究已经阐明了再生的大部分特征。一种称为组织工程的新技术，涉及从分离的细胞、生物相容性支架旧物和生长因子形成的结构中形成新组织。在本研究中，我们使用 MSC 作为细胞来源、陶瓷和可吸收材料。胶原蛋白作为基质，rhBMP-2 和富血小板血浆作为生长因子为了检验培养骨再生在临床上的有效性，我们研究了以下五件事：1) 基质的发育，2) 体内成骨。 ，3）建立更大规模的成骨细胞培养方法，4）生长因子促进骨形成的有效性5）在人体中的临床应用的可能性对于基质，我们研究了成骨作用。不可吸收的HA和新型可生物降解的β-磷酸三钙（β-TCP）的潜力，表明β-TCP将是骨再生的理想支架。此外，我们发现可吸收的胶原蛋白将是rhBMP的有效支架。 - 2 在窦和自体骨中然而，大多数关于骨形成的研究都采用二维组织形态学评估和分子生物学，而不是诸如显微CT的三维。我们还成功地评估了人类临床病例中骨小梁的微观结构，从现在开始，我们将进行研究，以提高牙周或牙槽缺损移植物在临床上使用间充质干细胞培养骨再生技术的实用性。具有可塑性。

项目成果

Minoru Ueda: "Hard and soft tissue engineering: applications in implant surgery"Tissues Engineering for Therapeu tic. 191-202 (2001)

Minoru Ueda：“硬组织工程和软组织工程：在种植手术中的应用”用于治疗的组织工程。

上田 実: "人体再生"現代医学. 49.1. 19-23 (2001)

上田稔：《人体再生》现代医学49.1。

上田 実: "ティッシュエンジニアリングの最新動向-幹細胞を利用した骨再生"The Quintessence Year Book 2001. 10-14 (2001)

Minoru Ueda：“组织工程的最新趋势 - 使用干细胞进行骨再生”The Quintessence Year Book 2001. 10-14 (2001)

山田陽一: "骨形成細胞による骨再生"炎症と免疫. 9.3. 3-9 (2001)

Yoichi Yamada：“成骨细胞的骨再生”炎症和免疫学 9.3（2001）。

上田 実: "ティッシュエンジニアリングの最新動向-幹細胞を利用した骨再生"The Quintessence Year Book 2001. 10-14 (2001)

Minoru Ueda：“组织工程的最新趋势 - 使用干细胞进行骨再生”The Quintessence Year Book 2001. 10-14 (2001)