Analysis of pathogenic mechanism of chronic pain in oro-facial aria using a index of CRF gene expression in hypothalamus

下丘脑CRF基因表达指数分析口面部慢性疼痛发病机制

• 批准号: 19592401
• 负责人: SHOJI Noriaki
• 金额: $ 2.16万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19592401/
• 关键词: 痛みストレス; 視床下部-下垂体-副腎系; CRF遺伝子; hnRNA; 視床下部-下垂体-副腎系(HPA axis); 急性痛; CRHmRNA; CRH hnRNA; Real-time PCR解析; 視床下部-下垂体-副腎系(HPA. axis); 慢性痛; ACTH; corticosterone; カプサイシン

Pain is one of the physical stressor for humans. In this study, we performed quantitative analysis of CRF gene transcription in hypothalamic PVN under the condition of tongue pain stress in rats. Plasma adrenocorticotropic hormone (ACTH) secretion was peak at 30 min after the capsaicin injection to tongue prior to gradually increasing of plasma corticosterone, indicating that tongue pain is stressor enough to up-regulate hypothalamo-pituitary- adrenocortical axis. CRF hnRNA 15 min after the injection was up-regulated 3 fold than that of control rats. This report is the first study which succeeded to detect CRF hnRNA by quantitative real-time PCR analysis.

疼痛是人类的身体压力之一。在这项研究中，我们对大鼠舌头疼痛应激的条件下，对下丘脑PVN中CRF基因转录进行了定量分析。血浆肾上腺皮质激素（ACTH）分泌在辣椒素注射后30分钟峰值在舌头上峰值，然后逐渐增加血浆皮质酮，表明舌头疼痛足以上调下调下丘脑甲状腺素 - 肾上腺皮质皮层轴。注射后15分钟的CRF HNRNA上调了3倍，比对照大鼠的CRF HNRNA。该报告是通过定量的实时PCR分析成功检测CRF HNRNA的第一项研究。

项目成果

Absence of sympathetic innervation and sprouting in rat dentinal tubules

大鼠牙本质小管缺乏交感神经支配和发芽

• 发表时间: 2008
• 作者: Yoichi Shimeno;Yumiko Sugawara;Masahiro Iikubo;Noriaki Shoji;Takashi Sasano
• 通讯作者: Takashi Sasano

歯科から心療内科に紹介された口腔心身症に関する臨床的考察-主体的な心身医学療法の復権を目指して-

牙医转介心身医学的口腔心身疾病的临床考虑 - 旨在恢复独立的心身医学治疗 -

• 发表时间: 2007
• 期刊: 日歯心身 22
• 作者: 杉本是明;庄司憲明;佐藤しづ子;笹野高嗣;中山孝子;杉本是孝
• 通讯作者: 杉本是孝