A study of the influence of occlusal activity on the characteristics of tongue and masticatory muscles using non-occlusal model animal

非咬合模型动物咬合活动对舌和咀嚼肌特征影响的研究

• 批准号: 19592266
• 负责人: ISHIKAWA Chieko
• 金额: $ 2.33万
• 依托单位: Tsurumi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19592266/
• 关键词: 小眼球症マウス; 咬合; シナプス形成; AChR転換; AChR除去; mitf; 咬筋; 二次元電気泳動; プロテオーム解析; 萌出不全; 筋形成; C2C12; 筋線維数; 筋線維断面積; MCK; siRNA; AChR; Real time PCR

The present study attempted to elucidate the contribution of occlusal activity to synaptogenesis in masticatory muscles. In the masseter of the mi/mi, the nAChR elimination initiated, but did not progress normally after 3 weeks of age, when the occlusal activity emerged in the +/+ mouse, whereas the nAChR switch progressed normally during the entire period of synaptogenesis. The mRNA expression patterns of nAChR subunits in the temporalis and digastric of the mi/mi differed from those in its masseter. These findings suggest that, in the masseter, occlusal activity is essential for the completion of nAChR elimination, but not for the nAChR switch, and that the contribution of occlusal activity to synaptogenesis varies among the masticatory muscles.

本研究试图阐明咬合活动对咀嚼肌突触发生的贡献。在 mi/mi 的咬肌中，nAChR 消除开始，但在 3 周龄后没有正常进展，此时 +/+ 小鼠出现咬合活动，而 nAChR 开关在整个突触发生期间正常进展。 mi/mi颞肌和二腹肌中n​​AChR亚基的mRNA表达模式与其咬肌中的不同。这些发现表明，在咬肌中，咬合活动对于完成 nAChR 消除至关重要，但对于 nAChR 转换则不然，并且咬合活动对突触发生的贡献在咀嚼肌之间存在差异。

项目成果

Function of occlusal activity in the synaptogenesis and myogenesis of masseter muscle

咬合活动在咬肌突触发生和肌生成中的作用

• 发表时间: 2008
• 作者: AMANE;A.;KOUTA;Y.;SUGA;T.;KANEKO;N.;MORITO;N. and ASADA;Y.
• 通讯作者: Y.