Effect of high fat diet on endurance capacity and oxydative enzyme activities in rat skeletal muscle.

高脂饮食对大鼠骨骼肌耐力和氧化酶活性的影响。

基本信息

批准号：
10680029
负责人：
NAKATANI Akira
金额：
$ 1.86万
依托单位：
Nara University of Education
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

The purpose of this study was to determine the effect of high fat diet on endurance capacity and oxidative enzyme activities in rat skeletal muscle.Male Wistar rats were assigned to either a high fat diet and a low fat diet group. They ate these diets ad libitum for 5 weeks. Although triceps muscle glycogen concentration in the high fat diet group was about half of that in the low fat diet group, swimming capacity was significantly higher in the high fat than in the low fat diet group. The high fat diet induced significant increases in citrate synthase (CS) and 3-hydroxyacyl CoA dehydrogenase (3-HAD) activities in triceps muscle (23% and 47%, respectively).Wistar rats were fed one of four diets : low fat (12% calories as fat) , lard (60% calories as fat) , safflower oil (60% calories as fat) , or fish oil (60% calories as fat). There were no significant differences in body weight among each diet group. Epidydimal adipose tissue weight was higher in lard and safflower oil groups than in low fat. Safflower and fish oil diet induced significant increases in CS and 3-HAD activities in red portion of gastrocnemius muscle.These results suggest that long term feeding of a high fat diet increases mitochondrial enzyme activities in skeletal muscle, and these increases might induce glycogen sparing and thus improve endurance.

本研究的目的是确定高脂肪饮食对大鼠骨骼肌耐力能力和氧化酶活性的影响。雄性Wistar大鼠被分配到高脂肪饮食组和低脂肪饮食组。他们随意食用这些饮食 5 周。尽管高脂饮食组的三头肌糖原浓度约为低脂饮食组的一半，但高脂饮食组的游泳能力明显高于低脂饮食组。高脂肪饮食诱导三头肌中柠檬酸合成酶 (CS) 和 3-羟酰基辅酶 A 脱氢酶 (3-HAD) 活性显着增加（分别为 23% 和 47%）。 Wistar 大鼠被喂食四种饮食之一：低脂（ 12% 卡路里为脂肪）、猪油（60% 卡路里为脂肪）、红花油（60% 卡路里为脂肪）或鱼油（60% 卡路里为脂肪）胖的）。每个饮食组之间的体重没有显着差异。猪油和红花油组的附睾脂肪组织重量高于低脂组。红花和鱼油饮食诱导腓肠肌红色部分的 CS 和 3-HAD 活性显着增加。这些结果表明，长期喂养高脂肪饮食会增加骨骼肌中的线粒体酶活性，这些增加可能会导致糖原节约和从而提高耐力。