Local action of inhibin dimmer and α-monomer

抑制剂二聚体和α-单体的局部作用

• 批准号: 10671514
• 负责人: ABE Yumiko
• 金额: $ 0.77万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671514/
• 关键词: Inhibin; Inhibin Dimer; α monomer; Activin; Follistatin

Inhibin dimmer inhibits FSH secretion from anterior pituitary cells in primary culture and inhibin α-monomer has no effect on FSH secretion. But as reported so far, the action of inhibin is different from tissue to tissue. In order to establish a suitable system to investigate the action of inhibin, we investigated several cells using activin because the availability of inhibin is very limited and inhibin and activin use common receptors. (1) Pituitary cells : The regulation of PRL secretion by activin from a rat pituitary cell line with type-I and type-II activin receptor complex on the cell surface was investigated. PRL secretion was changed by activin. Further study regarding the involvement of the inhibin/activin signal in PRL production is useful for our study. (2) Ovarian cells : Granulosa cells from DES-primed immature rats and oocyte-granulosa-theca cell complex from immature mice reacted to activin in low concentrations. Therefore these cells seem to be suitable in order to study the action of inhibin dimmer and α-monomer. (3) Placental cells : A primary culture of human placental cells at term was investigated. Only high concentrations of activin changed the secretion of hCG so these cells do not seem suitable to use to investigate the local action of inhibin.

抑制素二聚体抑制原代培养物中垂体前叶细胞的 FSH 分泌，而抑制素 α-单体对 FSH 分泌没有影响，但据迄今为止报道，抑制素的作用因组织而异，因此需要建立合适的系统进行研究。为了研究抑制素的作用，我们使用激活素研究了几种细胞，因为抑制素的可用性非常有限，并且抑制素和激活素使用共同的受体（1）垂体细胞​​：的调节。研究了细胞表面具有 I 型和 II 型激活素受体复合物的大鼠垂体细胞系中激活素的 PRL 分泌情况。有关抑制素/激活素信号参与 PRL 产生的进一步研究正在进行中。 (2) 卵巢细胞：来自 DES 引发的未成熟大鼠的颗粒细胞和来自未成熟小鼠的卵母细胞-颗粒-卵泡膜细胞复合体对激活素有低反应。因此，这些细胞似乎适合研究抑制素二聚体和 α-单体的作用。 (3) 胎盘细胞：研究了足月时人胎盘细胞的原代培养物仅改变了激活素的分泌。 hCG 因此这些细胞似乎不适合用于研究抑制剂的局部作用。

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