Protective effect of ischemic preconditioning (IPC) in ischemia-induced reperfusion injury of moto-organs

缺血预处理（IPC）对运动器官缺血再灌注损伤的保护作用

• 批准号: 10671380
• 负责人: YOKOYAMA Kazuhiko
• 金额: $ 1.86万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671380/
• 关键词: Ischemic preconditioning; Reperfusion injury; Skeletal muscle; Histochemical analysis; Necrosis; Ischemic Precoditioning; 再潅流障害; 組織化学的分析; 運動器; 虚血後再灌流障害; ischemic preconditioning; 一酸化窒素; フリーラジカル

Objective: Ischemic preconditioning (IPC) implies that repeated brief episodes of ischemia followed by reperfusion to tissue results in improved tissue survival when its tissue is subsequently subjected to prolonged ischemia. The aim of the present study is to determine if the number of cycles influences the effectiveness of IPC in skeletal muscle ischemia. Materials and Methods: using a total of 41 rat hindlimbs, the hindlimb-reperfusion model was created by temporary cutting both soft-tissue and bony structures, exclusive of femoral vessels, around the thighs, and clamping the vessels with a microvascular clip. The setting of each IPC cycle was determined to be 10 minutes of ischemia followed by 10 minutes of reperfusion. All animals were devided into seven groups: sham controls (SC) group (n=8); the same surgical procedures with no ischemia and no IPC, IR group (n=4); 4-hr global ischemia following no IPC, IPC-1, 2 group (n=7), and IPC-3, 4, 5 (n=5); 4-hr ischemia following 1 cycle … More to 5 cycles of IPC in each IPC group. Each animal was sacrificed after 24-hr reperfusion following the completion of each surgical and clamping procedure, the viability of its anterior tibial muscle was histochemically evaluated using NBT method, and the average necrosis area ratio (%NAR) of each group was caltulated by degitalized computer technique using NIH Image Soft. The %NAR was compared between groups using ANOVA and post hoc test, and each value was expressed as Mean±SEM.. Results: The %NAR of each group was as follows: SC group; 0±0, IR group; 66.2±5.9, IPC-1 group; 27.5±8.0, IPC-2 group; 29.5±6.2, IPC-3 group; 9.6±3.4, IPC-4 group; 10.3±2.2, and IPC-5 group ; 11.4±2.4. The %NARs of all IPC groups were significantly lower than that of IR group (p<0.0001). The %NAR of IPC-3, 4, and 5 were significantly lower, when compared with those of IPC-I and IPC-2 groups (p<0.01). Conclusion: The appropriate cycle number of IPC in skeletal muscle was clarified to be 3 to 5 cycles on the basis of the present study. The future studies are planed to study chemical and pathological evidence on the effectiveness of IPC. Less

目的：缺血预适应（IPC）意味着重复短暂的缺血发作后对组织进行再灌注，当组织随后遭受长期缺血时，可以提高组织的存活率。本研究的目的是确定周期数是否会影响组织的存活率。 IPC 在骨骼肌缺血中的有效性 材料和方法：使用总共 41 只大鼠后肢，通过临时切割软组织和骨来创建后肢再灌注模型。将每个 IPC 周期的设置确定为 10 分钟缺血，然后 10 分钟再灌注。对照组（SC）（n = 8）；相同的手术程序，无缺血且无 IPC，IR 组（n = 4），无 IPC 后出现 4 小时全身缺血； IPC-1、2 组（n=7）和 IPC-3、4、5（n=5）；每个 IPC 组在 1 个周期至 5 个周期后出现 4 小时缺血。每次手术和钳夹完成后24小时再灌注，使用NBT方法对其胫前肌的活力进行组织化学评估，并计算平均坏死面积率（％NAR）使用NIH Image Soft通过数字化计算机技术计算各组的％NAR，并使用ANOVA和事后检验比较组间的％NAR，并将每个值表示为Mean±SEM。 结果：各组的％NAR如下： SC组；0±0，IR组；66.2±5.9，IPC-1组；27.5±8.0，IPC-2组； IPC-3组；9.6±3.4，IPC-4组；10.3±2.2，IPC-5组；11.4±2.4，所有IPC组的%NAR均显着低于IR组（p<0.0001）。与IPC-I和IPC-2组相比，IPC-3、4和5组的NAR显着降低(p<0.01)：根据本研究，骨骼肌中 IPC 的适当周期数已明确为 3 至 5 个周期，未来的研究计划研究 IPC 有效性的化学和病理证据。较少的

项目成果

横山一彦: "Effect of coenzyme Q_ on superoxide production in rats with reperfusion injury."Scand. J. Plast. Reconstr. Hand. Surg.. 33. 1-5 (1999)

Kazuhiko Yokoyama：“辅酶 Q_<10> 对再灌注损伤大鼠中超氧化物产生的影响。”Scand. Reconstr. 33. 1-5 (1999)

Nakamura Ko, Yokoyama K, Nakamura K, Itoman M: "Chronological changes in nitric oxide, superoxide, and blood circulation in muscles after warm ischaemic.reperfusion. -Using a model of rabbit rectus femoris muscle."Scand. J. Plast. Reconstr. Hand Surg.. 33

Nakamura Ko、Yokoyama K、Nakamura K、Itoman M：“热缺血再灌注后肌肉中一氧化氮、超氧化物和血液循环的时间变化。-使用兔股直肌模型。”扫描。

中村光伸: "Superoxide generation in reperfusion after arterial ischemia and venous congestion" British Journal of Plastic Surgery. 51. 633-636 (1998)

Mitsunobu Nakamura：“动脉缺血和静脉充血后再灌注中的超氧化物生成”英国整形外科杂志 51. 633-636 (1998)。

横山一彦: "Effect of coenzyme Q10 on superoxide production in rats with reperfusion injury"Scand. J. Plast. Reconstr. Hand. Surg.. 33. 1-5 (1999)

Kazuhiko Yokoyama：“辅酶 Q10 对再灌注损伤大鼠中超氧化物产生的影响”，J. Reconstr. 33. 1-5 (1999)

Yokoyama K, Kimura M, Nakamura K, Nakamura Ko, Itoman M: "Time course of post-ischemic superoxide generation in venous effluent from reperfused rabbit hindlimb."J. Reconstr. Microsurg. 15(3). 215-221 (1999)

Yokoyama K、Kimura M、Nakamura K、Nakamura Ko、Itoman M：“再灌注兔后肢静脉流出物中缺血后超氧化物生成的时间过程。”J。