Clinical significance and regulatory mechanisms of Mcl-1 in esophageal squamous cell carcinoma

Mcl-1在食管鳞癌中的临床意义及调控机制

• 批准号: 19590728
• 负责人: ISOMOTO Hajime
• 金额: $ 2.66万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19590728/
• 关键词: Mcl-1; STAT3; microRNA; epigenetics; SOCS3; microRNA-205; microRNA-10a; 上皮間葉転換; squamous cell carcinoma; esophagus; micro RNA; miR-205; miR-29b; 食道扁平上皮癌; stat3; IL-6; miRNA-205; JAK

We sought to identify esophageal squamous cell carcinoma (SCC) specific microRNA (miR) to elucidate regulatory mechanisms of Mcl-1. RNA from SCC cell lines, immortalized non-malignant SC cell and various malignant cells was subjected to miR microarray. The candidate miRs were analyzed by quantitative RT-PCR. MiR-205 can be specifically upregulated in the SCC. As for its function, miR-205 was associated with migration capacity with Matrigel assay, but not with proliferation, apoptosis and wound healing, when assessed by cellular transfection of its precursor or inhibitor. On the other hand, miR-10a was substantially downregulated in the SCC, and could be restored by treatment with 5-azadeoxycytidine and/or trichostatin A.

我们试图鉴定食管鳞状细胞癌（SCC）特异性microRNA（miR）以阐明MCL-1的调节机制。 SCC细胞系，永生的非恶性SC细胞和各种恶性细胞的RNA受到miR微阵列。通过定量RT-PCR分析候选miR。 miR-205可以在SCC中明确上调。至于其功能，当通过细胞转染其前体或抑制剂的细胞转染评估时，miR-205与Matrigel测定法的迁移能力与增殖，凋亡和伤口愈合无关。另一方面，MiR-10a在SCC中大大下调，可以通过5-氮杂氧基丁胺和/或Trichostatin A进行恢复。

项目成果

miRNA-205 modulates cellular invasion and migration via regulating zinc finger

miRNA-205通过调节锌指调节细胞侵袭和迁移

• 发表时间: 2009

miRNA205とmiRNA 10aは食道扁平上皮癌に特異的マーカーと成りうるか?

miRNA205和miRNA 10a能否作为食管鳞癌的特异性标志物？

• 发表时间: 2008

miRNA205とmiRNA 10aは食道扁平上皮癌に特異的である

miRNA205和miRNA 10a对食管鳞状细胞癌具有特异性

• 发表时间: 2009

miRNA205とmiRNA 10aは食道扁平上皮癌に特異的マーカーである

miRNA205和miRNA 10a是食管鳞状细胞癌的特异性标志物

• 发表时间: 2009
• 作者: 磯本 一;松島加代子;井上直樹
• 通讯作者: 井上直樹