Pure isolation of nitrite-oxidizing bacteria from several environments and development of new phylogenetic marker for them.

从多种环境中纯分离亚硝酸盐氧化细菌并为其开发新的系统发育标记。

• 批准号: 19580096
• 负责人: TAKAHASHI Reiji
• 金额: $ 2.33万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19580096/
• 关键词: 環境微生物学; 亜硝酸酸化菌; 分子系統分類; 亜硝酸酸化還元酵素遺伝子; nxrB; 新規分離; 分布解析; 微生物群集構造解析; DGGE; Nitrobacter; norB

Nitrite oxidizing bacteria (NOB) that inhabit the whole environment extensively are bacterial species that are in charge of the latter part of nitrification. But little strains of them are isolated as pure culture. This study were carried out for exploration and its application of useful of genetic resources and showed following knowledge. First, as many NOB strains were newly isolated from a variety of environments, we got sufficient strains for studying microbial properties of them. Second, we explored new markers for phylogenetic classification for NOB. Third, we established a new phylogenetic classification with new markers, and we have applied it to "non-culture" analysis of NOB in natural environments.

广泛存在于整个环境中的亚硝酸盐氧化细菌（NOB）是负责硝化后期的细菌种类。但其中很少有菌株被作为纯培养物分离出来。本研究是为了探索遗传资源的有用性及其应用而进行的，得出了以下认识。首先，由于许多NOB菌株是从各种环境中新分离出来的，我们获得了足够的菌株来研究它们的微生物特性。其次，我们探索了 NOB 系统发育分类的新标记。第三，我们用新的标记建立了新的系统发育分类，并将其应用于自然环境中NOB的“非培养”分析。

项目成果

タイ王国酸性硫酸塩土壌から分離されたアンモニア酸化菌のウレアーゼの発現解析

泰国酸性硫酸盐土壤氨氧化细菌脲酶表达分析

• 发表时间: 2008
• 作者: 日比野倫士;高橋令二;中川達功;佐藤一朗;徳山龍明
• 通讯作者: 徳山龍明

Characteristics of newly isolated ammonia-oxidizing bacteria from acid sulfate soil and the rhizoplane of leucaena grown in that soil.

酸性硫酸盐土壤中新分离的氨氧化细菌及其生长的银合欢根面的特征。

• 发表时间: 2007
• 期刊: Soil Sci. Plant Nutr 53
• 作者: Satoh;K.;Itoh;C.;Kang;D.-J.;Sumida;H.;Takahashi;R.;Isobe;K.;Sasaki;S.;Tokuyama T
• 通讯作者: Tokuyama T

Analysis of ammonia monooxygenase and archaeal 16S rRNA gene fragments in nitrifying acid-sulfate soil microcosms.

• DOI: 10.1264/jsme2.me09104
• 发表时间: 2009-06
• 期刊: Microbes and environments
• 影响因子: 2.2
• 作者: Asami Nakaya;Y. Onodera;T. Nakagawa;Kazuo Satoh;R. Takahashi;S. Sasaki;T. Tokuyama

Seasonal change in vertical distribution of ammonia-oxidizing archaea and bacteria and their nitrification in temperate forest soil.

• DOI: 10.1264/jsme2.me09179
• 发表时间: 2010-03
• 期刊: Microbes and environments
• 影响因子: 2.2
• 作者: Y. Onodera;T. Nakagawa;R. Takahashi;T. Tokuyama

各種環境より分離した亜硝酸酸化菌の特性と新規分類指標の検討

不同环境中亚硝酸氧化菌的特性及新分类指标的检验

• 发表时间: 2007
• 作者: 高橋令二;中川達功;徳山龍明
• 通讯作者: 徳山龍明