DEVELOPMENT OF CELL HYBRID ARTIFICIAL BONE USING 3-D CELL CULTURE SYSTEM

使用 3-D 细胞培养系统开发细胞混合人工骨

• 批准号: 08557114
• 负责人: ASAHINA Izumi
• 金额: $ 4.93万
• 依托单位: TOKYO MEDICAL AND DENTAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08557114/
• 关键词: ARTIFICIAL BONE; BMP; FGF; BONE MARROW CELL; HYDROXYAPATITE; ハイブリッド; bFGF; 再生; 成長因子

(1) Effects of Bone Morphogenetic Protein (BMP and basic Fibroblast Growth Factor (bFGF) on Bone Marrow Cel1s : We examined the effects of BMP and bFGF on rat bone marrow cells, and revealed that BMP stimulated both cell proliferation and differentiation, while bFGF stimulated cell proliferation but inhibited cell differentiation. bFGF, especially, enhanced the proliferation of osteoprogenitor cells, which had not diffeientiated into matures osteoblast yet. Therefor, the treatment of bFGF at the early stage of osteoblastic differentiation increased the. number of osteoprpgenitor cells and the treatment of BMP at the later stage induced differentiation of osteoblast. As a result the treatment of both BMP and bFGF at the optimal stage of osteoblastic differentiation promoted osteogenesis.(2) Fabrication of Cell Hybrid Artificial Bone : New bone formation was induced in rat subcutaneous tissue by implanted bone marrow cells cultured in porous hydroxyapatite. The treatment of BMP and/or bEOF during the culture in vitro enhanced bone formation in vivo. We also examined porous poly (gycoic, lactic acid co-polymer) as a scaffold for 3-D cell culture, but cells did not infiltrate deeply into the block because of it7s hydrophobicity.

（1）骨形态发生蛋白（BMP和碱性成纤维细胞生长因子（bFGF）对骨髓细胞的影响：我们检测了BMP和bFGF对大鼠骨髓细胞的影响，发现BMP刺激细胞增殖和分化，而bFGF bFGF 刺激细胞增殖，但抑制细胞分化，特别是增强尚未分化为成熟成骨细胞的骨祖细胞的增殖。在成骨细胞分化的早期阶段使用bFGF可以增加骨祖细胞的数量，而在后期使用BMP可以诱导成骨细胞的分化，因此在成骨细胞分化的最佳阶段使用BMP和bFGF都可以促进成骨。 (2)细胞混合人工骨的制造：通过植入在多孔羟基磷灰石中培养的骨髓细胞，在大鼠皮下组织中诱导新骨形成。体外培养期间的bEOF增强体内骨形成。我们还研究了多孔聚（藻酸、乳酸共聚物）作为 3-D 细胞培养的支架，但由于其疏水性，细胞并未深入渗透到块中。

项目成果

I Asahina,et.al: "Repair of Bone Defect in Primate Mandible using a Bone Morphogenetic Protein (BMP)-Hydroxyapatite-Collagen Composite" J.Med.Dent.Sci.44・3. 63-70 (1997)

I Asahina 等人：“使用骨形态发生蛋白（BMP）-羟基磷灰石-胶原复合物修复灵长类下颌骨的骨缺损”J.Med.Dent.Sci.44·3（1997）。

I Asahina, TK Sampath PV Hauschka: "Human Osteogenic Protein-1 Induces Chondroblastic, Osteoblastic, and/or Adipocytic Differentiation of Clonal Murine Target Cells" Experimental Cell Research. 222. 38-47 (1996)

I Asahina，TK Sampath PV Hauschka：“人类成骨蛋白-1 诱导克隆小鼠靶细胞的软骨细胞、成骨细胞和/或脂肪细胞分化”实验细胞研究。

M Higashinakagawa: "Biological Effects of Basic Fibroblast Growth Factor (bFGF) and Osteogenic Protein-1 (OP-1/BMP-7) on Bone Marrow Cells and Development of a Cell Hybrid Artificial Bone" J Stomatological Society.(in press). (1999)

M Higashinakakawa：“碱性成纤维细胞生长因子（bFGF）和成骨蛋白-1（OP-1/BMP-7）对骨髓细胞和细胞混合人工骨发育的生物效应”口腔医学会杂志（出版中）。

S Oida,et.al: "Biological Activities of Bone Extract containing TGF-β superfamily proteins" J.Hard Tiss.Biol.7・1. 27-36 (1998)

S Oida等人：“含有TGF-β超家族蛋白的骨提取物的生物活性”J.Hard Tiss.Biol.7·1（1998）。

Isobe M and Yamazaki Y Oida S,Ishihara K., Nakabayashi N,Amagasa, T.: "Bone morphogenetic protein encapsulated with a biodegradable and biocompatible polymer." J.Biomed.Mater.Res.32. 433-438 (1996)

Isobe M 和 Yamazaki Y Oida S、Ishihara K.、Nakabayashi N、Amagasa、T.：“用可生物降解和生物相容性聚合物封装的骨形态发生蛋白。”