Enhancement of apoptosis in oral cancer cells by intracellular reactive oxygen amplication

细胞内活性氧扩增增强口腔癌细胞凋亡

• 批准号: 18592214
• 负责人: WADA Shigehito
• 金额: $ 2.43万
• 依托单位: University of Toyama
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18592214/
• 关键词: Hyperthermia; Radiation; Oxidative stress; Oral cancer; Reactive oxygen; 活性酸素増幅法; 細胞内酸化ストレス

The effects of various free radicals derived from 6-formylpterin (6-FP), alpha-phenyl-tert-butyl nitrone (PBN) and 2,2-azobis (2-amidinopropane) dihydrochloride (AAPH) combined with hyperthermia, on gene expression in similarly enhanced apoptosis of human lymphoma U937 cells were investigated using cDNA microarrays containing approximately 16,600 genes and computational gene expression analysis tools. When the cells were treated for 10 min at 44 degrees C (15% apoptosis level), 39 up-regulated and 3 down-regulated genes were identified. In the up-regulated genes, apoptosis-and unfolded protein response-associated genes were contained. The combined treatment with heat and either chemical enhanced apoptosis level (approximately 30%) and showed a chemical-specific gene expression pattern. Furthermore, the expression levels of selected genes were confirmed by a real-time quantitative PCR. The present results will provide a basis for further understanding the molecular mechanisms in enhancement of heat-induced apoptosis by different intracellular oxidative stress.

6-甲酰蝶呤（6-FP）、α-苯基叔丁基硝酮（PBN）和2,2-偶氮二（2-脒基丙烷）二盐酸盐（AAPH）衍生的各种自由基与热疗联合对基因表达的影响使用包含约 16,600 个基因的 cDNA 微阵列和计算基因表达分析工具，研究了人淋巴瘤 U937 细胞的类似增强凋亡。当细胞在 44 摄氏度（15% 凋亡水平）下处理 10 分钟时，鉴定出 39 个上调基因和 3 个下调基因。在上调的基因中，包含与凋亡和未折叠蛋白反应相关的基因。热和任一化学物质的联合处理增强了细胞凋亡水平（约30%），并显示出化学物质特异性的基因表达模式。此外，通过实时定量PCR确认了所选基因的表达水平。本研究结果将为进一步了解不同细胞内氧化应激增强热诱导细胞凋亡的分子机制提供基础。

项目成果

Pulp ablation therapy by inductive heating-heat generation characteristics in pulp cavity

感应加热牙髓消融治疗——牙髓腔发热特性

• 发表时间: 2007
• 期刊: Oral Diseases
• 影响因子: 3.8
• 作者: Wada;S.;Tazawa;K.;Suzuki;N.;Furuta;I.;Nagano;I
• 通讯作者: I

Pulp ablation therepy by inductive heating-heat generation character istics in pulp cavity-.

感应加热牙髓消融治疗-牙髓腔内发热特性-。

• 发表时间: 2007
• 期刊: Oral Diseases 13
• 作者: Wada S.;et. al.
• 通讯作者: et. al.

A hydrogen peroxide-generating agent, 6-formylpterin, enhances heat-induced apoptosis

• DOI: 10.1080/02656730400025404
• 发表时间: 2005-05
• 期刊: International Journal of Hyperthermia
• 影响因子: 3.1
• 作者: S. Wada;Zheng-Guo Cui;Takashi Kondo;Qing‐Li Zhao;R. Ogawa;M. Shoji;Toshiyuki Arai;Keisuke Makino;Isao Furuta

Gene exoression ln enhanced apoptosis of human lyphoma U937 cells treated with the combination of different free radical generators and hyperthermia

不同自由基发生器和热疗联合处理的人淋巴瘤U937细胞凋亡中的基因外排增强

• 发表时间: 2007
• 期刊: Free radical research 41
• 作者: Wada S.;et. al.
• 通讯作者: et. al.

Gene expression in enhanced apoptosis of human lymphoma U937 cells treated with the combination of different free radical generators and hyperthermia

不同自由基发生器和热疗联合处理人淋巴瘤U937细胞凋亡增强中的基因表达

• 发表时间: 2007
• 期刊: Free Radical Res 41
• 作者: Wada;S.;Tabuchi;Y.;Kondo;T.;Cui;Z-G.;Zhao;Q-L.;Takasaki;I.;Salunga;TL.;Ogawa;R.;Arai;T.;Makino;K.;Furuta;I
• 通讯作者: I