Enhancement of apoptosis in oral cancer cells by intracellular reactive oxygen amplication

细胞内活性氧扩增增强口腔癌细胞凋亡

• 批准号: 18592214
• 负责人: WADA Shigehito
• 金额: $ 2.43万
• 依托单位: University of Toyama
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18592214/
• 关键词: Hyperthermia; Radiation; Oxidative stress; Oral cancer; Reactive oxygen; 活性酸素増幅法; 細胞内酸化ストレス

The effects of various free radicals derived from 6-formylpterin (6-FP), alpha-phenyl-tert-butyl nitrone (PBN) and 2,2-azobis (2-amidinopropane) dihydrochloride (AAPH) combined with hyperthermia, on gene expression in similarly enhanced apoptosis of human lymphoma U937 cells were investigated using cDNA microarrays containing approximately 16,600 genes and computational gene expression analysis tools. When the cells were treated for 10 min at 44 degrees C (15% apoptosis level), 39 up-regulated and 3 down-regulated genes were identified. In the up-regulated genes, apoptosis-and unfolded protein response-associated genes were contained. The combined treatment with heat and either chemical enhanced apoptosis level (approximately 30%) and showed a chemical-specific gene expression pattern. Furthermore, the expression levels of selected genes were confirmed by a real-time quantitative PCR. The present results will provide a basis for further understanding the molecular mechanisms in enhancement of heat-induced apoptosis by different intracellular oxidative stress.

衍生自6种甲甲基蛋白（6-FP），α-苯基 - 苯基 - 二丁基硝酸（PBN）和2,2-氮杂（2-2-氮杂烷）二羟基氯化物（AAPH）在类似的高温表达中与人类lymthercome seply cdne copta米其度相结合的各种自由基的影响。包含大约16,600个基因和计算基因表达分析工具。当细胞在44度C（15％凋亡水平）下处理10分钟时，鉴定出39个上调和3个下调基因。在上调的基因中，凋亡和未折叠的蛋白质反应相关基因被包含。用热量和化学化学凋亡水平（约30％）进行了联合处理，并显示出化学特异性基因表达模式。此外，通过实时定量PCR证实了选定基因的表达水平。目前的结果将为进一步理解通过不同细胞内氧化应激增强热诱导凋亡的分子机制提供基础。

项目成果

Pulp ablation therapy by inductive heating-heat generation characteristics in pulp cavity

感应加热牙髓消融治疗——牙髓腔发热特性

• 发表时间: 2007
• 期刊: Oral Diseases
• 影响因子: 3.8
• 作者: Wada;S.;Tazawa;K.;Suzuki;N.;Furuta;I.;Nagano;I
• 通讯作者: I

Pulp ablation therepy by inductive heating-heat generation character istics in pulp cavity-.

感应加热牙髓消融治疗-牙髓腔内发热特性-。

• 发表时间: 2007
• 期刊: Oral Diseases 13
• 作者: Wada S.;et. al.
• 通讯作者: et. al.

A hydrogen peroxide-generating agent, 6-formylpterin, enhances heat-induced apoptosis

• DOI: 10.1080/02656730400025404
• 发表时间: 2005-05
• 期刊: International Journal of Hyperthermia
• 影响因子: 3.1
• 作者: S. Wada;Zheng-Guo Cui;Takashi Kondo;Qing‐Li Zhao;R. Ogawa;M. Shoji;Toshiyuki Arai;Keisuke Makino;Isao Furuta

Gene exoression ln enhanced apoptosis of human lyphoma U937 cells treated with the combination of different free radical generators and hyperthermia

不同自由基发生器和热疗联合处理的人淋巴瘤U937细胞凋亡中的基因外排增强

• 发表时间: 2007
• 期刊: Free radical research 41
• 作者: Wada S.;et. al.
• 通讯作者: et. al.

Gene expression in enhanced apoptosis of human lymphoma U937 cells treated with the combination of different free radical generators and hyperthermia

不同自由基发生器和热疗联合处理人淋巴瘤U937细胞凋亡增强中的基因表达

• 发表时间: 2007
• 期刊: Free Radical Res 41
• 作者: Wada;S.;Tabuchi;Y.;Kondo;T.;Cui;Z-G.;Zhao;Q-L.;Takasaki;I.;Salunga;TL.;Ogawa;R.;Arai;T.;Makino;K.;Furuta;I
• 通讯作者: I