Development research into molecular target diagnosis and treatment method of cancer of oral cavity that makes Wnt signaling factor target

以Wnt信号因子为靶点的口腔癌分子靶向诊疗方法的开发研究

• 批准号: 18592183
• 负责人: KOBAYASHI Masashi
• 金额: $ 2.53万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18592183/
• 关键词: cancer of oral cavity; diagnosis; treatment; Wnt signaling; β-catenin; cancer of oral cavity; diagnosis; treatment; Wnt signaling; β-catenin

It is thought that the cancer of oral cavity is still feared as a fatal disease ease regardless of remarkable advancement of the diagnosis and the treatment method of the cancer of oral cavity and a big obstacle is given from the change to a morbid state of the face it to QOL. Little research of the relation between the cancer of oral cavity and the Wnt cell signaling route is advanced though β-Catenin is thought to be one of the oncogenes. It was thought that it was necessary to promote this research in the emergency for overcoming at the early stage of the cancer of oral cavity, examined as follows, and it reaped the benefit.1. Examination of irregular accumulation to cytoplasm and nucleus of B-Catenin of culture mouth cancer cell.Both what β-Catenin has accumulated abnormally in the cytoplasm and the nucleus and the thing that had not accumulated the label by the fluorescent antibody to recognize β-Catenin of the culture flatness cuticle cancer cell and the culture saliva glandular carcinoma cell, the observation with the electron microscope, and the retrieval whether β-Catenin had accumulated abnormally in the cytoplasm and the nucleus existed.2. Examination of gene mutation of β-Catenin of culture mouth cancer cell. RNA of β-Catenin of the culture flatness cuticle cancer cell and the culture saliva glandular carcinoma cell was extracted, the presence of the gene mutation was retrieved by the PCR-SSCP method, direct sequence method Denco mutation was tried in addition, and the decision of the position was tried. In the culture mouth cancer cell that had abnormally accumulated β-Catenin in the cytoplasm and the nucleus, the one with the gene mutation of β-Catenin and the one that not was existed. Moreover, the area was a part phosphorylated by GSK3.

人们认为，无论诊断的明显进步和口腔癌的治疗方法如何，口腔癌仍然被错误地担心，并且口腔癌的治疗方法是从面孔到质量杂质的摩尔比德状态的大障碍。对口腔癌与Wnt细胞信号传导途径之间关系的研究很少，认为β-catenin被认为是其中一种癌基因。据认为，有必要在紧急情况下促进这项研究，以克服口腔癌的早期，如下所示，并获得了收益。1。 Examination of irregular accumulation to cytoplasm and nucleus of B-Catenin of culture mouth cancer cell.Both what β-Catenin has been accumulated significantly in the cytoplasm and the nucleus and the thing that had not accumulated the label by the fluorescent antibody to recognize β-Catenin of the culture flatness cuticle cancer cell and The culture saliva glandular carcinoma cell, the observation with the electron microscope, β-catenin是否在细胞质和核中显着积累了检索。2。检查培养口腔癌细胞的β-catenin的基因突变。提取了培养扁平性切口癌细胞和培养唾液癌细胞的RNA，提取了通过PCR-SSCP方法检索基因突变的存在，并尝试了直接序列方法Denco突变，并尝试了该位置的决策。在培养的口腔癌细胞中，绝对积累了细胞质和细胞核中的β-catenin，具有β-catenin的基因突变，而不存在的基因突变。此外，该区域是由GSK3磷酸化的一部分。