The study on the cells forming periodontium by observing the expression of dentin proteins as the markers of differentiation
以牙本质蛋白表达作为分化标志物研究牙周形成细胞
基本信息
- 批准号:18591996
- 负责人:
- 金额:$ 1.93万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2006
- 资助国家:日本
- 起止时间:2006 至 2007
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Dentin sialoprotein (DSP) was first elucidated as a dentin specific protein and has been thought to be a marker of odontoblast and preameloblast. Since we have already detected the expression of DSP in periodontium during its formation, we supposed that the cells forming periodontium might originate from those differentiate into odontoblasts. The purpose of this project using rat mandibular first molar (M1) was to define the cells forming periodontium by observing the expression of the dentin proteins as the markers of those differentiation. We also utilized the 5-bromo2'-deoxy-uridine (BrdU) to observed proliferation and migration of the cells forming periodontium.While the results of this projected study could not show the clear evidence to define proliferation and migration of the cells forming periodontium, we found the interesting activity of dental pulp cells during root formation. In the animals at post natal 1 d in which dentin matrix formation has just started, a few BrdU incorporating cells were detected at crown pulp area, while positive cells were rarely detected in the animals at 5 d and 7 d. In 10 d, root dentin formation has started and was going on 14 d-old. BrdU labels suggesting cell proliferation were detected at dental pulp around the apical formation front of root dentin. At 21-old, BrdU label was again rarely detected in dental pulp. Combined with the previous reports showing cell proliferation in developing dental papilla, our results suggested "2 step proliferation" of dental papilla and/or pulp cells, one step is for crown part and the other is for root.
首先将牙本质唾液蛋白(DSP)阐明为牙本质特异性蛋白质,被认为是牙糖细胞和前素细胞的标志。由于我们已经在其形成过程中检测到DSP在牙周地区的表达,因此我们认为形成牙周的细胞可能源自那些分化为牙糖细胞。该项目使用大鼠下颌第一磨牙(M1)的目的是通过观察牙本质蛋白作为这些分化的标记来定义形成牙周的细胞。我们还利用了5-Bromo2'-脱氧 - 尿苷(BRDU)来观察到形成牙周的细胞的增殖和迁移。这项预计的研究的结果无法证明明确的证据以定义形成细胞的增殖和迁移,这些细胞形成了牙周的细胞,但我们发现牙髓形成过程中牙髓细胞的有趣活性。在牙本质基质形成刚刚开始的纳塔尔后1天的动物中,在冠状果肉区域中检测到一些掺入细胞的BRDU,而在5 d和7 d的动物中很少在动物中检测到阳性细胞。在10 d中,根牙本质的形成已经开始,并且正在14 d大。 BRDU标签表明在根牙本质的顶端形成前面检测到牙浆中检测到细胞增殖。在21岁时,在牙纸浆中很少检测到BRDU标签。结合先前的报道显示了发育中的牙齿乳头上的细胞增殖,我们的结果表明牙皮乳头和/或纸浆细胞的“ 2步增殖”,一个步骤是冠状部分,而另一步是针对根。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
An immunohistochemical and ultrastructural study of the pericellular matrix of uneroded hypertrophic chondrocytes in the mandibular condyle of aged c-src-deficient mice
老年 c-src 缺陷小鼠下颌骨髁中未侵蚀肥大软骨细胞周基质的免疫组织化学和超微结构研究
- DOI:
- 发表时间:2008
- 期刊:
- 影响因子:0
- 作者:Tomokiyo A;他5名;Shinichi Shibata
- 通讯作者:Shinichi Shibata
Diverse effects of c-src deficiency on molar tooth development and eruption in mice
c-src 缺乏对小鼠臼齿发育和萌出的多种影响
- DOI:
- 发表时间:2007
- 期刊:
- 影响因子:0
- 作者:Niyata A;Baba O;Oda T;Ishikawa I;Takano Y.
- 通讯作者:Takano Y.
The formation of periodontium during long term organ culture of mouse tooth germ
小鼠牙胚长期器官培养过程中牙周组织的形成
- DOI:
- 发表时间:2007
- 期刊:
- 影响因子:0
- 作者:Tearashima T;Baba O;Shikano S;Abe T;Yamashita Y;Oida S
- 通讯作者:Oida S
Immunohistochemical study on root formation of mouse tooth germ during long term organ culture
长期器官培养过程中小鼠牙胚根形成的免疫组织化学研究
- DOI:
- 发表时间:2006
- 期刊:
- 影响因子:0
- 作者:Tearashima T;Baba O;Oida S;TakanoY
- 通讯作者:TakanoY
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