Novel Roles of mast cells in Eosinophilic nasal polyps including aspirin sensitive nasal polyps

肥大细胞在嗜酸性鼻息肉（包括阿司匹林敏感鼻息肉）中的新作用

基本信息

批准号：
18591889
负责人：
PAWANKAR Ruby
金额：
$ 2.43万
依托单位：
Nippon Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591889/
关键词：
Nasal Polyps Mast cell MC(T) and MC(TC)FcεRI Eosinophils MMP-9 IL-13 IgE AIA IL-4,IL-13 AIAに伴う鼻茸 TLR

项目摘要

The mechanisms of eosinophilic nasal polyps and aspirin induced nasal polyps is not well defined. Most studies have focused on investigating the epithelial cells and eosinophilic mediators. Mast cells are increased in the nasal polyps and are important inflammatory cells and are particularly increased in eosinophilic nasal polyps and aspirin sensitive nasal polyps and may play a role in the infiltration of eosinophilsIn the present study, we used the the mast cell culture system we established to investigate the activating mechanisms of the nasal polyp mast cells. Upon stimulating nasal polyp mast cells with the FceRI antibody we investigated the levels of histamine, tryptase, IL-4, IL-5 TNF-alpha, IL-13 and TGF-beta by ELISA. Histamine, tryptase, IL-, IL-5, IL-13, TNF-alpha were increased in the cell supernatants of stimulated mast cells.The levels of histamine and eosinophilic cationic protein that are proteins of mast cells and eosinophils respectively gad a good correlation. From the point of view of increased IL-4 and IL-13, these are related to the increased levels of IgE in nasal polyps. Histaimine from nasal polyp mast cells upregulates RANTES production from nasal epithelial cells and this in turn can induce increased eosinophil infiltration into the nasal polypMMP-9 also was increased in nasal polyp. TIMP-1 and TIMP-2 were less. Mast cells and eosinophils exprrssed MMP-9. This can induce the structural changes seen in eosinophilic nasal polyps

嗜酸粒细胞性鼻息肉和阿司匹林诱发鼻息肉的机制尚不明确。大多数研究都集中在研究上皮细胞和嗜酸性介质。肥大细胞在鼻息肉中增多，是重要的炎症细胞，在嗜酸性粒细胞和阿司匹林敏感鼻息肉中尤其增多，可能在嗜酸性粒细胞浸润中发挥作用。在本研究中，我们使用我们建立的肥大细胞培养系统研究鼻息肉肥大细胞的激活机制。用 FceRI 抗体刺激鼻息肉肥大细胞后，我们通过 ELISA 研究了组胺、类胰蛋白酶、IL-4、IL-5 TNF-α、IL-13 和 TGF-β 的水平。受刺激的肥大细胞的细胞上清液中组胺、类胰蛋白酶、IL-、IL-5、IL-13、TNF-α增加。组胺和嗜酸性阳离子蛋白（分别是肥大细胞和嗜酸性粒细胞的蛋白质）的水平具有良好的相关性。从IL-4和IL-13升高来看，这些与鼻息肉中IgE水平升高有关。鼻息肉肥大细胞中的组胺上调鼻上皮细胞中 RANTES 的产生，这反过来又会诱导鼻息肉中嗜酸性粒细胞浸润增加 pMMP-9 在鼻息肉中也有所增加。 TIMP-1和TIMP-2较少。肥大细胞和嗜酸性粒细胞表达MMP-9。这可以引起嗜酸性鼻息肉的结构变化