Potential therapeutic utility of an anti-angiogenic factor, pigment epithelium-derived factor for the treatment of osteosarcoma

抗血管生成因子、色素上皮衍生因子治疗骨肉瘤的潜在治疗效用

• 批准号: 18591648
• 负责人: YAMAGISHI Sho-ichi
• 金额: $ 2.57万
• 依托单位: Kurume University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591648/
• 关键词: pigment epithelium-derived factor; peptide; osteosarcoma; angiogenesis

Angiogenesis is required for tumor growth and progression. We have recently found that pigment epithelium-derived factor (PEDF) inhibits growth of both endothelial cells and tumor cells in vitro, thus suggesting that PEDF could exert anti-tumor effects. In this project, we investigated which PEDF domains could have anti-angiogenic properties both in vitro and in vivo. In vitro, three PEDF peptides (amino acids 381-387, 388-393, 394-400) were found to have anti-angiogenic effects on endothelial cells; their anti-angiogenic potencial was similar to that of PEDF full protein. Further, the peptides had growth inhibitory effects on osteosarcoma cells. In vivo, the peptides were also found to suppression angiogenesis elicited by alkaline injury in cornea. The present study demonstrated that the PEDF peptides had anti-angiogenic and tumorstatic effects on osteosarcoma cells. The observations suggest the potential therapeutic utility of the PEDF peptides for the treatment of osteosarcoma.

血管生成是肿瘤生长和进展所必需的。我们最近发现，色素上皮因子（PEDF）在体外抑制了内皮细胞和肿瘤细胞的生长，因此表明PEDF可以发挥抗肿瘤作用。在这个项目中，我们研究了哪些PEDF结构域在体外和体内都可能具有抗血管生成特性。在体外，发现三种PEDF肽（氨基酸381-387，388-393，394-400）对内皮细胞具有抗血管生成作用。它们的抗血管生成势力与PEDF全蛋白相似。此外，肽对骨肉瘤细胞具有生长抑制作用。在体内，还发现肽是角膜中碱性损伤引起的血管生成。本研究表明，PEDF肽对骨肉瘤细胞具有抗血管生成和肿瘤的作用。观察结果表明，PEDF肽对骨肉瘤的治疗潜在的治疗效用。

项目成果

Pigment epithelium-derived factor inhibits neointimal hyperplasia after vascular injury by blocking NADPH oxidase-mediated reactive oxygen species generation

• DOI: 10.2353/ajpath.2007.060838
• 发表时间: 2007-06-01
• 期刊: AMERICAN JOURNAL OF PATHOLOGY
• 影响因子: 6
• 作者: Nakamura, Kazuo;Yamagishi, Sho-ichi;Imaizumi, Tsutomu
• 通讯作者: Imaizumi, Tsutomu

Pigment epithelium-clerived factor inhibits neointimal hyperplasia after vascular injury by blocking NADPH oxidase-mediated reactive oxygen species generation

色素上皮细胞分泌因子通过阻断 NADPH 氧化酶介导的活性氧生成来抑制血管损伤后的新生内膜增生

• 发表时间: 2007
• 期刊: Am J Pathology 170
• 作者: Nakamura K;et. al.
• 通讯作者: et. al.

Pigment epithelium-derived factor(PEDP) administration inhibits occlusive thrombus formation in rats : a possible participation of reduced intraplatelet PEDF in thrombosis of acute coronary syndrome

色素上皮衍生因子（PEDP）给药抑制大鼠闭塞性血栓形成：血小板内PEDF减少可能参与急性冠脉综合征血栓形成

• 发表时间: 2008
• 期刊: Atherosclerosis 197
• 作者: Takenaka K;et. al.
• 通讯作者: et. al.

Administration of pigment epithelium-derived factor (PEDF) inhibits cold injury-induced brain edema in mice

• DOI: 10.1016/j.brainres.2007.04.088
• 发表时间: 2007-09-05
• 期刊: BRAIN RESEARCH
• 影响因子: 2.9
• 作者: Jinnouchi, Yuko;Yamagishi, Sho-ichi;Imaizumi, Tsutomu
• 通讯作者: Imaizumi, Tsutomu

Pigment epithelium-derived factor (PEDF) administration inhibits occlusive thrombus formation in rats:a possible participation of reduced intraplatelet PEDF in thrombosis of acute coronary syndrome

色素上皮衍生因子（PEDF）给药抑制大鼠闭塞性血栓形成：血小板内PEDF减少可能参与急性冠脉综合征血栓形成

• 发表时间: 2008
• 期刊: Atherosclerosis 197
• 作者: Takenaka K;et. al.
• 通讯作者: et. al.