INTERPOSITION NERVE GRAFTS TO RESTORE POSTOPERATIVE URINARY AND SEXUAL FUNCTIONS IN RATS.

插入神经移植物以恢复大鼠术后泌尿和性功能。

• 批准号: 07671350
• 负责人: KONO Toru
• 金额: $ 1.47万
• 依托单位: ASAHIKAWA MEDICAL COLLEGE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671350/
• 关键词: cancer of the rectum; autonomous nerve preservation operation; interposition nerve grafts auto-transplantation; tetanus toxin C fragment; bladder function; 骨盤内臓神経; 腓腹神経; 骨盤内自律神経機能; 神経自家移植; 骨盤内蔵神経; 自律神経機能温存; 神経細胞追跡標識物質; 免疫神経組織化学的手法; 電気生理学的手法

AimBy surgery treatment of cancer of the rectum discovered early comparatively, the autonomous nerve preservation operation in the pelvis which considered function maintenance after operation is recommended, but there is the case that curability of cancer is thought about in the advanced case, and cannot but abandon preservation operation currently. This study is a fundamental study to make clear the propriety of new operation method development letting a function after advaned cancer operation maintains by using an interposition nerve grafts auto-transplantation.Methods and Results(1) In the graft group, tetanus toxin C fragment (transsynaptic retrograde transporter) was apllied to the bladder wall with a purpose to confirm the regeneration which was occured via the grafted nerve. After 2 or 3 weeks, parasympathetic nerve cells in lower spinal cord (S2-4) were retrogradely stained by tetanus toxin C fragment via the transplanted and reproducted nerve.(2) After 2 or 3 weeks, the recovered bladder function wasn't recognized.(3) After 5 or 6 weeks, the function recovery preservation was recognized and was almost near by bladder function in normality.SummaryI was able to suggest the possibility that an interposition nerve grafts auto-transplantation was extremely useful for function recovery after operation in advanced rectal cancer patients.

目的通过比较早期发现的直肠癌的手术治疗，推荐考虑术后功能维持的骨盆自主神经保留手术，但存在晚期病例考虑癌症的治愈性而不得不放弃的情况当前保存操作。本研究是一项基础研究，旨在明确开发新的手术方法的合理性，使晚期癌症手术后通过自体神经移植物维持功能。方法和结果（1）在移植物组中，破伤风毒素C片段（将跨突触逆行转运蛋白）应用于膀胱壁，目的是确认通过移植神经发生的再生。 2或3周后，破伤风毒素C片段通过移植和再生的神经对下脊髓（S2-4）的副交感神经细胞进行逆行染色。（2）2或3周后，膀胱功能未恢复。 (3) 5或6周后，功能恢复保留得到确认，几乎接近正常的膀胱功能。总结我能够建议插入神经移植的可能性自体移植对于晚期直肠癌患者术后功能恢复非常有用。

项目成果

柿坂明俊、他: "回腸ストーマ症例における回腸組織大腸化と腸内細菌叢の変化に関する検討" 日臨外医会誌. 57(1). 23-29 (1996)

Akitoshi Kakizaka 等人：“回肠造口病例中回肠组织定植和肠道菌群变化的研究”日本门诊学会杂志 57（1）（1996）。

Kono, T., et al.: "Spontaneously‐occurred inducible nitric oxide synthase (iNOS) in adenoma and adenocarcinoma in human colon." The Biology of Nitric Oxide Part 5 Portland Press. 4. 154- (1996)

Kono, T., 等人：“人类结肠腺瘤和腺癌中自发发生的诱导型一氧化氮合酶 (iNOS)。一氧化氮生物学第 5 部分，波特兰出版社 (Portland Press)。

Kono,T.,et al.: "Nitric Oxide (NO) is released from liver into the abdominal cavity in the acut liver failure animal model." FASEBJ.10(3). A705 (1996)

Kono,T.,et al.：“在急性肝功能衰竭动物模型中，一氧化氮 (NO) 从肝脏释放到腹腔中。”

Kono, T., Iwamoto, J., Y,Yamamoto, Kakisaka, A., Kasai, S., and M.Mito.: "Nitric Oxide (NO) is released from liver into the abdominal cavity in the acute liver failure animal model.19GC01 : FASEB J." 10 (3). A705 (1996)

Kono, T.、Iwamoto, J.、Y、Yamamoto、Kakisaka, A.、Kasai, S. 和 M.Mito.：“在急性肝衰竭动物中，一氧化氮 (NO) 从肝脏释放到腹腔中

Yamamoto,Y.,et al.: "Effect of low-power laser irradiation on procollagen synthesis in human fibrobasts." J.Clin.Laser Med.Surg.14. 129-132 (1996)

Yamamoto,Y.,et al.：“低功率激光照射对人成纤维细胞中前胶原合成的影响。”