Study of cell regulation by sodium sensor channels

钠传感器通道的细胞调节研究

• 批准号: 18500251
• 负责人: WATANABE Eiji
• 金额: $ 2.57万
• 依托单位: National Institute for Basic Biology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18500251/
• 关键词: glia; sodium; sensor; homeostasis; channel; circumventricular organs; gene-targetine mouse; cell culture

Sodium homeostasis is crucial for life and Na-levels in body fluids are constantly monitored in the brain. The subfornical organ (SFO) is the center of the sensing responsible for the control of Na-intake behavior, where Nax channels are expressed in specific glial cells as the sodium-level sensor. Here, we show direct interaction between Nax channels and subunits of Na+/K+-ATPase, which brings about Na-dependent activation of the metabolic state of the glial cells. The metabolic enhancement leading to extensive lactate production was observed in the SFO of wild-type mice, but not of the Nax-knockout mice. Furthermore, lactate, as well as Na, stimulated the activity of GABAergic neurons in the SFO. These results suggest that the information on a physiological increase of the Na level in body fluids sensed by Nax in glial cells is transmitted to neurons by lactate as a mediator to regulate neural activities of the SFO.

钠稳态对于生命至关重要，体液中的NA水平在大脑中不断监测。亚圆形器官（SFO）是负责控制Na-Intake行为的感应的中心，其中NAX通道在特定的神经胶质细胞中表示为钠级传感器。在这里，我们显示了Na+/K+-ATPase的NAX通道和亚基之间的直接相互作用，这带来了神经胶质细胞代谢态的Na依赖性激活。在野生型小鼠的SFO中观察到了代谢增强，导致广泛的乳酸产生，但没有在NAX-敲除小鼠中观察到。此外，乳酸以及Na刺激了SFO中GABA能神经元的活性。这些结果表明，关于神经胶质细胞中NAX感测的Na水平的生理升高的信息通过乳酸作为调节SFO神经活性的介体传播到神经元。

项目成果

脳のナトリウムレベルセンサー〜遺伝子改変マウスによる解析〜

脑钠水平传感器~使用转基因小鼠进行分析~

• 发表时间: 2007
• 作者: Shimizu;H.;Watanabe;E.;Hiyama;T.Y.;Nagakura;A.;Fujikawa;A.;Noda;M.;Ayano Nagakura;Hidetada Shimizu;渡辺 英治
• 通讯作者: 渡辺 英治

脳のナトリウムセンサー〜グリアは脳の情報処理解明のブレイクスルーに成り得るか?〜

大脑中的钠传感器：神经胶质细胞能否成为阐明大脑信息处理的突破？

• 发表时间: 2007
• 作者: Watanabe;E.;渡辺 英治
• 通讯作者: 渡辺 英治

Characterization of neurons in the mouse subfbrnical organ by retrograde labeling

通过逆行标记表征小鼠毛下器官中的神经元

• 发表时间: 2007
• 作者: Shimizu;H.;Watanabe;E.;Hiyama;T.Y.;Nagakura;A.;Fujikawa;A.;Noda;M.;Ayano Nagakura
• 通讯作者: Ayano Nagakura

Before and After sodium sensor

钠传感器之前和之后

• 发表时间: 2008
• 作者: Watanabe;E.
• 通讯作者: E.

Sodium-level sensor of the brain

大脑的钠水平传感器

• 发表时间: 2007
• 作者: Watanabe;E.
• 通讯作者: E.