Regeneration of resorbed tooth roots by cementogenesis

通过牙骨质形成再生吸收的牙根

基本信息

批准号：
18390552
负责人：
SUDA Naoto
金额：
$ 11.01万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18390552/
关键词：
root resorption cementoblast regeneration amelogenin emdogain odontoclast 成長因子破歯細胞

项目摘要

Pathological tooth root resorption is frequently seen with infection, trauma and tumor, and also induced by some dental treatments. Prevention of pathological resorption in Permanent teeth is a crucial issue to avoid tooth loss and maintain normal oral function, however, there is no effective approach or management for it, so far.Emdogain gelR is a porcine enamel matrix derivative which can promote periodontal tissue regeneration. Since amelogenin is known as one of the bioactive substances in Emdogain, it is quite interesting to ask whether amelogenin and Emdogain have an inhibitory action on odontoclastic root resorption. To clarify this point; we examined the effect of amelogenin on odontoclast formation, in vitro. Furthermore, rat molars were replanted and the root resorption was induced. Emdogain was treated and the effect, on root resorption was examined, in vivo.Odontoclastic cells were isolated from human deciduous teeth and cultured in the presence of 1α25(OH)2vitaminD3 (Vit. D3). The upper first molars off 6 week-old male rats were extracted, and teeth were left on the bench for 1 hr after periodontal ligaments were removed Each tooth was replanted into the socket Root surfaces of some teeth were treated with Emdogain gelR or emdogain powder mixed with the propylene glycol alginate before the replantation. Rats were sacrificed 7 days after replantation and serial saggital sections were prepared.1) P172 treatment significantly decreased odontoclastic Dell number in the culture.2) Numerous root resorption lacunae and odontoclasts were seen on the root surface of replanted teeth. In the experimental group, in which Emdogain gelR or Emdogain powder was applied on roots, values of the odontoclast surface, odontoclast number, and the resorbed dentin and cementum were all significantly lower than those of untreated roots.As conclusion, all these findings suggest that amelogenin and Emdogain inhibit the root resorption, in vitro and in vivo.

病理性牙根吸收常见于感染、创伤和肿瘤，也由一些牙科治疗引起，预防恒牙病理性吸收是避免牙齿脱落和维持正常口腔功能的关键问题，但目前尚无有效的方法。 Emdogain gelR是一种猪牙釉质基质衍生物，可以促进牙周组织再生，因为amelogenin被认为是Emdogain的生物活性物质之一，所以问这个问题很有趣。牙釉蛋白和 Emdogain 是否对牙根吸收有抑制作用为了澄清这一点，我们在体外检查了牙釉蛋白对牙根吸收的影响。，在体内检查牙根吸收。破牙细胞细胞从人乳牙中分离出来，并在存在以下物质的情况下培养： 1α25(OH)2维生素D3 (Vit.D3) 拔除6周龄雄性大鼠的上第一磨牙，去除牙周韧带后将牙齿留在工作台上1小时，将每颗牙齿重新种植到牙槽窝的根面中。在再植后7天处死大鼠之前，用与海藻酸丙二醇混合的恩多加因凝胶R或恩多加因粉末处理一些牙齿。制备再植牙和连续矢状切片。1）P172处理显着降低了培养物中的破牙细胞数量。2）再植牙牙根表面可见大量牙根吸收陷窝和破牙细胞。将粉末涂在根部，破牙细胞表面、破牙细胞数量、吸收牙本质和牙骨质的值均显着低于未处理的根。总之，所有这些发现表明，amelogenin 和 Emdogain 在体外和体内抑制根吸收。