Molecular mechanisms controlling circadian rhythms and photoperiodism in insects

控制昆虫昼夜节律和光周期的分子机制

• 批准号: 18380043
• 负责人: TAKEDA Makio
• 金额: $ 11.8万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18380043/
• 关键词: 光周性; 概日振動; メラトニン合成系; 光受容; 変態; 休眠; 表現型多型; N-acetyltransferease (NAT); メラトニン; long wave opsin; action sp[ectra; 卵休眠; Cycle; 概日時計; NAT; serotonin; 5HT receptor; UVB; opsin; マダラスズ; pars intercerebralis; 摂食; セロトニン; N-アセチル転移酵素(NAT); 神経分泌

Photoperiodism is an important biological mechanism and involved in agriculture production but its molecular mechanism remains obscure.This study analyzed the structures, localization and dynamics of the elements involved in photoreception, circadian oscillation and endocrine switch to reconstruct the photoperiodic system. Comparative studies using different insects revealed a considerable flexibility in the circadian pacemaker structure and photoreceptor regarding to ontogenetic stages and phylogenetic lines. The important factor mediating photpoperiodic system seems to be the indolaminergic pathway, since the upstream regulatory region of N-acetyltransferase(NAT) that catalyze the production of melatonin has several E-boxes where transcription factors CLK/CYK binds, the cells secreting prothoracicotropic horone (PTTH) express melatonin receptor and all PER containing cells have NAT, and melatonin content and NAT transcript level show a circadian oscillation. These cells have the extraretinal photoreception.

光周期是一种重要的生物学机制，并参与农业生产，但其分子机制仍然晦涩难懂。这项研究分析了涉及光感受，昼夜节律振荡和内分泌切换以重建光周期系统的结构，定位和动态。使用不同昆虫的比较研究表明，昼夜节律起搏器结构和感光器具有相当大的灵活性，涉及个体发生阶段和系统发育线。 The important factor mediating photpoperiodic system seems to be the indolaminergic pathway, since the upstream regulatory region of N-acetyltransferase(NAT) that catalyze the production of melatonin has several E-boxes where transcription factors CLK/CYK binds, the cells secreting prothoracicotropic horone (PTTH) express melatonin receptor and all PER containing cells have NAT, and褪黑激素含量和NAT转录水平显示出昼夜节律。这些细胞具有视野外感受。

项目成果

Molecular characterization and distribution of cycle protein from Athalia rosae

玫瑰花循环蛋白的分子特征和分布

• 发表时间: 2007
• 期刊: J. Insect Physiol. 53
• 作者: Bembenek J;Itokawa K;Hiragaki S.;ShaoQ.M;Tufail M.;Takeda M.
• 通讯作者: Takeda M.

Casein kinase I in the Silkworm, Bombyx mori : Structure and possible roles in circadian timing and development

家蚕中的酪蛋白激酶 I：结构及其在昼夜节律和发育中的可能作用

• 发表时间: 2006
• 期刊: J. Biol. Rhythms 21
• 作者: Le Thi Dieu T;Sehadova H.;Ichihara N.,Iwai S. Mita K;Takeda M.
• 通讯作者: Takeda M.

Co-localization and unique distributions of two clock proteins CYCLE and CLOCK in the cephalic ganglia of the ground cricket

两种时钟蛋白 CYCLE 和 CLOCK 在地蟋蟀头神经节中的共定位和独特分布

• 发表时间: 2008
• 期刊: Allonemobius allardi Cell and Tissue Research 331
• 作者: Shao;Q-M;Hiragaki;S;Takeda;M
• 通讯作者: M

Takeda M. (2009)Putative regulatory mechanism of prothoracicotropic hormone (PTTH) secretion in the American cockroach, Periplaneta americana as inferred from co-locatization of Rab8, PTTH and protein kinase C in neurosectretory cells.

Takeda M. (2009) 根据神经分泌细胞中 Rab8、PTTH 和蛋白激酶 C 的共定位推断美洲大蠊 (Periplaneta americana) 促促胸腺激素 (PTTH) 分泌的假定调节机制。

• 发表时间: 2009
• 期刊: Cell and Tissue Research 335
• 作者: Hiragaki;S.;Uno T.;Takeda M.
• 通讯作者: Takeda M.

Takeda M. Molecular cloning and expression analysis of two putative serotonin receptors in the brain of Antheraea pernyi pupa.

武田 M. 柞蚕蛹大脑中两种假定的血清素受体的分子克隆和表达分析。

• 期刊: International Journal of Wild Sikmoths and Silk
• 作者: Hiragaki;S.;Kawabe Y.
• 通讯作者: Kawabe Y.