Role of free radicals on the reperfusion injury of ischemic heart -study with radical scavengers-

自由基对缺血性心脏再灌注损伤的作用-自由基清除剂的研究-

• 批准号: 07670806
• 负责人: KATAGIRI Takashi
• 金额: $ 1.41万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670806/
• 关键词: Ischemia-Reperfusion; Free radial; ESR; Cerium chloride; Catalase; Sarcoprasmic reticulum; Mitochondria; ACE inhibitor; Ca-ATPase; Hydrogen peroxide; Superoxide dismutase; 再潅流障害; フリーラジカル; SOD; catalase; working heart; cerium chloride

Object :We studied the role of free radicals on the reperfusion injury in the various experimental models of ischemic heart.Results :1. We could detect the free radicals with electron spin resonance (ESR) method using spin trapping agent, POBN,in the rat and dog models of ischemia-reperfused heart from 30 sec to 5 min after reperfusion.2. Ca^<++>-ATPase activity of sarcoplasmic reticulum (SR) and its composing protein was earlier degradated in the ischemia-reperfused heart than ischemia-nonreperfused heart in the rat and dog models.3. In the perfused rat heart and isolated myocyte, free radical generations from mitochondria and endothelial cell were detected visually using Cerium chloride, reactant to the hydrogenperoxide, with ultrastructural observation.4. In the stunned heart of the dog model (15 min ischemia followed by 60 min reperfusion), contractile function and its SR function were more improved with treatment of superoxide dismutase (SOD) and ACE-inhibitor (alacepril).Conclusion :Free radial generation was one of the initial factors of the reperfusion injury.

目的：研究自由基对不同缺血性心脏实验模型再灌注损伤的作用。结果： 1．利用自旋捕获剂POBN，采用电子自旋共振(ESR)法，在大鼠和犬缺血再灌注心脏模型再灌注后30秒至5分钟内检测到自由基。 2．大鼠和狗缺血再灌注心脏中肌浆网(SR)的Ca^++-ATPase活性及其组成蛋白比未再灌注心脏更早降解。 3．在灌注的大鼠心脏和分离的心肌细胞中，使用过氧化氢的反应物氯化铈，通过超微结构观察，肉眼检测线粒体和内皮细胞产生的自由基。4．在狗的心脏电晕模型中（15 分钟缺血，然后 60 分钟再灌注），超氧化物歧化酶（SOD）和 ACE 抑制剂（阿拉普利）治疗后，收缩功能及其 SR 功能得到更大改善。结论：自由径向生成是再灌注损伤的初始因素之一。

项目成果

Ryuji Ueda,et al.: "Histochemical Demonstration of Hydrogen Peroxide Generation in Reperfused Rat Heats" VIII Biennial Meeting International Society for Free Radical Research,Abstract book. 153-3 (1996)

Ryuji Ueda 等人：“再灌注大鼠发热中过氧化氢生成的组织化学演示”第八届国际自由基研究学会双年会，摘要书。

Katsumichi Iijima: "Dose ACE-I (alacepril) Scarvenge Free Radicals on Stunned Myocardium?" VIII Biennial Meeting International Society for Free Radical Research,Abstract book. 152-3 (1996)

Katsumichi Iijima：“给昏迷的心肌服用 ACE-I（阿拉普利）清除自由基吗？”

Ryuji Ueda,et al.: "Production of Hydrogen Peroxide during Hypoxia-regenerated in Isolated Myocytes." J Mol Cell Cardiol. 28. A316 (1996)

Ryuji Ueda 等人：“离体心肌细胞缺氧再生期间过氧化氢的产生”。

Ryuji Ueda,et al.: "Histochemical Demonstration of Hydrogen Peroxide Generation in Endothelial Cells and Mitochondria in Rat Heart." Jpn CircJ. 60. 431-432 (1996)

Ryuji Ueda 等人：“大鼠心脏内皮细胞和线粒体中过氧化氢生成的组织化学演示。”

Ryuji Ueda,et al.: "Histochemical Demonstration of Hydrogen Peroxide Generation in Reperfused Rat Hearts" VIII Biennial Meeting International Society for Free Radical Research,Abstract book. 153-3 (1996)

Ryuji Ueda 等人：“再灌注大鼠心脏中过氧化氢生成的组织化学演示”第八届国际自由基研究学会双年会，摘要书。