Studies on the environmental control for the mass propagation of the tissue cultured transplants by direct ex-virto rooting method

组培直接离体生根法大量繁殖的环境控制研究

基本信息

批准号：
07660349
负责人：
HAYASHI Makio
金额：
$ 1.47万
依托单位：
Tokai University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

项目摘要

In this study, the environmental control for the mass propagation of the tissue culture plantlets by 'direct ex-vitro rooting method' was discussed. In the direct ex-vitro rooting method, the shoot elongation, rooting, and acclimatization of the explants/plantlets are simultaneously achieved in the environmental control unit under the photoautotrophic condition. The following results were taken from the experiments.1) The effects of the photosynthetic photon flux density (PPFD) and concentration oi culture solution on the growth of strawberry plantlets were examined. The explants of micropropagated shoots were directly inserted into rockwool plugs and cultured inside the environmental control unit by hydroponics using a culture solution at different concentrations. The effect of the concentration of culture solution on the growth of plantlets was obvious in high PPFD than that in low PPFD.2) The transpiration rate of the explants/plantlets was changed by wind speed at a certain high relative humidity. In order to reduce the death rate and to increase the growth rate of the plantlets, the control of the wind speed near plantlets seems to be as important as the control of humidity.3) The conditions to repress the death of plantlets were discussed and the various informations were obtained.4) The relationship between the weight of explants and the growth after planting was examined in this method. The relative growth rate (RGR) was promoted in the treatment of relatively low weight of the explants. It seems that the culture period will be shortened and small transplant will be produced in this method than in the conventional method.

本研究探讨了“直接离体生根法”组培苗大量繁殖的环境控制。在直接离体生根方法中，在光自养条件下，在环境控制单元中同时实现外植体/幼苗的芽伸长、生根和驯化。实验结果如下：1)考察了光合光子通量密度(PPFD)和培养液浓度对草莓试管苗生长的影响。将微繁芽的外植体直接插入岩棉塞中，并使用不同浓度的培养液通过水培法在环境控制单元内培养。培养液浓度对试管苗生长的影响在高PPFD下比在低PPFD下明显。2)在一定的高相对湿度下，风速改变了外植体/试管苗的蒸腾速率。为了降低试管苗的死亡率，提高试管苗的生长速度，控制试管苗附近的风速似乎与控制湿度一样重要。 3)讨论了抑制试管苗死亡的条件，并提出了控制试管苗附近风速的方法。获得了各种信息。4)该方法考察了外植体重量与种植后生长之间的关系。在外植体重量相对较低的处理中，相对生长率（RGR）得到提高。与传统方法相比，该方法似乎可以缩短培养周期并且可以产生小移植物。