INVESTIGATION ABOUT THE MECHANISM OF RETINAL ADHESION AND ITS BREAKDOWN AND RECOVERY

视网膜粘连及其破坏和恢复机制的研究

基本信息

批准号：
07457413
负责人：
NEGI Akira
金额：
$ 4.8万
依托单位：
KUMAMOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

项目摘要

1 Healing process of the rabbit retinal pigment epithelium after mechanical removal A dome shaped retinal detachment was created in the rabbit eye by injecting saline into the subretinal space. RPE under the detached retina was removed from Bruck's membrane about 1.5mm in diameter and the healing process was observed morphologically. Shortly after the injury, RPE cells around the wound started to fill the defected area by sliding and migrating, and after 2 weeks the wound was completely covered. In eyes with overlying sensory retina, regenerated RPE cells formed a monolayr similar to normal RPE.The microvilli and RPE-photoreceptor outer segment interface was reorganized. In eyes with no sensory retina, the regenerated RPE were multilayred and the microvilli were short and disorganized. In many cases of damaged Bruck's membrane, choroidal neovascularization was observed.2 Sealing process of the retinal hole in the rabbit eyeRetinal hole of 0.5mm in diameter was made in the rabbit eye. After making artificial posterior vitreous detachment, vitrectomy and gas tamponade were performed. One week after surgery, the retinal hole was perfectly closed in all eyes macroscopically.Histologically, the hole was filled with glial cells, fibroblast and RPE.Diaminido yellow staining revealed that glial cells accumulated actively to the retinal wound and this process playd important roll to seal the hole.3 Effects of steroid on the subretinal fluid resorptionExperimental serous detachment was made by injecting rose bengal and mild photocoagulation in the rabbit eye. Preliminary results showed the tendency that in a group of administrating steroid, the serous detachment survived longer than that in non-administered group.

1 机械去除兔视网膜色素上皮后的愈合过程通过向视网膜下腔注射生理盐水，在兔眼中形成圆顶形视网膜脱离。将脱离的视网膜下的RPE从Bruck膜上取下，直径约1.5mm，形态学观察愈合过程。受伤后不久，伤口周围的RPE细胞开始通过滑动和迁移来填充缺损区域，2周后伤口被完全覆盖。在感觉视网膜上层的眼睛中，再生的RPE细胞形成类似于正常RPE的单层。微绒毛和RPE-感光器外节界面被重组。在没有感觉视网膜的眼睛中，再生的 RPE 是多层的，微绒毛短且杂乱。在许多布鲁克膜受损的病例中，观察到脉络膜新生血管形成。2兔眼视网膜孔的封闭过程在兔眼上制作直径0.5mm的视网膜孔。人工玻璃体后脱离后，进行玻璃体切除术和气体填充。术后一周，肉眼观察，所有眼睛的视网膜裂孔均已完全闭合。组织学上，裂孔内充满了胶质细胞、成纤维细胞和RPE。二氨基黄染色显示，胶质细胞向视网膜创面积极聚集，这一过程对视网膜创面的修复起到了重要作用。 3 类固醇对视网膜下液吸收的影响通过兔眼注射孟加拉玫瑰花并轻度光凝进行实验性浆液性脱离。初步结果表明，在给予类固醇的组中，浆液脱离的存活时间比未给予组的要长。