Study on the mode of human parvovirus B19 infection

人细小病毒B19感染模式的研究

基本信息

批准号：
18591194
负责人：
YOTO Yuko
金额：
$ 2.57万
依托单位：
Sapporo Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

Human parvovirus B19 (B19) is known as causative agents of erythema infectiosum in children and aplastic crisis in patients with congenital chronic hemolytic anemia. B19 is believed to have a single immunoserotype individuals who have suffered a disease induced by B19 aquire lifelong immunity and won't usually be reinfected. As a rare occurrence, several patients with repeated B19 infection have been reported We encountered a patient who appeared to have reinfection 3 years after the primary infection. There was no difference in genome type between the primary and the secondary infection when serum samples from our patient who seemed to have a reinfection were analyzed and compared. Latent B19 infection may persist after a primary infection. The pathological mechanism of a latent B19 infection involved in such cases will be studied There may be a special strain which predisposes to latent infections. I have examined 9,000 serum samples from 1995 to 2005. Thirty samples were positive for B19 DNA. The nudeotides sequences are now being analyzed. The association between the nucleotides and symtoms are going to be examined. After this analysis, the artificial mutation will be made in the plasmide with B19 sequences and the mode of infection will be studied by investigating for respective mRNA transcripts of B19. Now I have found the special mutation of Thymidine to Adenine at 1969nt in the case of latent infection shown above. The other analysis is now being performed.

人类细小病毒 B19 (B19) 被认为是儿童传染性红斑和先天性慢性溶血性贫血患者再生障碍性危象的病原体。 B19被认为具有单一免疫血清型，患有由B19引起的疾病的个体获得终生免疫力，并且通常不会再次感染。作为一种罕见的情况，有几例重复感染 B19 的患者已被报道。我们遇到了一名患者，他在初次感染 3 年后似乎再次感染。当分析和比较似乎再次感染的患者的血清样本时，原发性感染和继发性感染之间的基因组类型没有差异。初次感染后，潜伏的 B19 感染可能会持续存在。将研究此类病例中涉及的潜伏性 B19 感染的病理机制。可能存在一种特殊的菌株，易于发生潜伏性感染。从 1995 年到 2005 年，我检查了 9,000 份血清样本。其中 30 份样本 B19 DNA 呈阳性。现在正在分析核苷酸序列。将检查核苷酸和症状之间的关联。分析后，将在具有 B19 序列的质粒中进行人工突变，并通过研究 B19 各自的 mRNA 转录本来研究感染模式。现在我发现在上面所示的潜伏感染的情况下，胸苷在1969nt处发生了特殊的腺嘌呤突变。目前正在进行另一项分析。