Identification of glucocorticoid target genes involved in visceral obesity

内脏肥胖相关糖皮质激素靶基因的鉴定

• 批准号: 18590997
• 负责人: KAJI Hidesuke
• 金额: $ 2.46万
• 依托单位: University of Hyogo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590997/
• 关键词: adipocyte; glucocorticoid; RNA interference (RNAi); metabolic syndrome (MetS); single nuculeotide polymorphism (SNP); CCAAT; enhancer binding protein 8 (C; EBPδ); neuropeptide Y (NPY); NPY type 2 receptor (NPY2R); ニューロペプチドY2受容体; メタボリックシンドローム

We investigated to clarify whether connective tissue growth factor, emerin, and calpain 1 are essential for the initial step of adipocyte differentiation, since glucocorticoid is very important for adipocyte differentiation, and mRNA expressions of these 3 molecules are induced by cocktail with dexamethasone during the initial adipocyte differentiation from 3T3-L1 cells, and they have their human ortholog as well as glucocorticoid responsive element on their 5'-flanking region of the gene. The RNA interference in each mRNA expression failed to change the adipocyte differentiation, suggesting that these molecules, at least by each single molecule, are not essential for the initial step of the adipocyte differentiation.Next, the association between metabolic syndrome (MetS) and CCAAT/enhancer binding protein δ (C/EBPδ) was investigated after informed consent in 172 persons for health checkup. It has been reported in the study using C/EBPδ knockout mouse that C/EBPδ mRNA induction by gluc … More ocorticoid during the initial adipocyte differentiation from 3T3-L1 cells is essential for visceral obesity. Our result indicated for the first time that genotype C/T in SNP rs15955 was more prevalent in dyslipidemia as well as impaired glucose tolerance (IGT) among phenotypes of MetS.Furthermore, neuropeptide Y (NPY)/NPY type 2 receptor (NPY2R) pathway is recently reported to be involved in high fat and sucrose diet-induced visceral obesity under certain stresses with stimulated glucocorticoid secretion. Therefore, we tested the association between MetS (IDF Asia criteria) and the 5'-flanking region of NPY2R gene SNPs with high minor allele frequency in Asian population. In 317 persons for health checkup participated in this study after informed consent, genotype '17 in SNP rs6857715 was more prevalent in MetS and IGT. Genotype AA in SNP rs6857530 was more prevalent in IGT. Further functional studies of the transcriptional control of these SNP regions will be required for the development of prevention strategy and drug discovery for MetS. Less

我们研究以阐明连接的组织生长因子，厄并蛋白和钙钙1是否对于脂肪细胞分化的初始步骤至关重要，因为糖皮质激素对于脂肪细胞的分化非常重要，并且这些3分子的mRNA表达是由鸡尾酒诱导的，在最初的脂肪细胞中，在3Tt3-tt3-tt3-tt3-tt3-tt3-tt3-tt3-tt3升的情况下诱导的mRNA表达是非常重要的。在基因的5'频乘区域上的响应元素。 The RNA interference in each mRNA expression failed to change the adipocyte differentiation, suggesting that these molecules, at least by each single molecule, are not essential for the initial step of the adipocyte differentiation.Next, the association between metabolic syndrome (MetS) and CCAAT/enhancer binding protein δ (C/EBPδ) was investigated after informed consent in 172 people for health checkup.在研究中使用C/EBPδ基因敲除小鼠据报道了C/EBPδMRNA通过Gluc诱导…在最初的脂肪细胞与3T3-L1细胞分化过程中更多的类齿型对于内脏肥胖至关重要。我们的结果表明，SNP RS15955中的基因型C/T首次在血脂异常中更普遍，并且在METTHERMORE的表型中，葡萄糖耐受性（IGT）受损。Furthermore，furthermore，Neuropeptide Y（NPY）/NPY 2受体（NPY2R）的较高含量（NPY 2R）在较高的情况下均在较高的情况下使用。刺激的糖皮质激素分泌的应力。因此，我们在亚洲人群中测试了MetS（IDF亚洲标准）与具有高次要等位基因频率的NPY2R基因SNP的5'Fanking区域之间的关联。在知情同意后，在317名进行健康检查的人中，SNP RS6857715中的基因型'17在MetS和IGT中更为普遍。 SNP RS6857530中的基因型AA在IGT中更为普遍。为了制定大都会的预防策略和药物发现，需要对这些SNP区域的转录控制进行进一步的功能研究。较少的

项目成果

A novel mutation in the von Hippel-Lindau tumor suppressor gene identified in a Japanese family with pheochromocytoma and hepatic hemangioma

在一个患有嗜铬细胞瘤和肝血管瘤的日本家族中发现了 von Hippel-Lindau 肿瘤抑制基因的新突变

• 发表时间: 2006
• 期刊: Intern Med 45・5
• 作者: Dai;G;Yamamoto D;Imanaka M;Takahashi K
• 通讯作者: Takahashi K

Genetic variations at the CCAAT/Enhancer-binding protein δ are associated with metabolic phenotypes in the Japanese population

CCAAT/增强子结合蛋白 δ 的遗传变异与日本人群的代谢表型相关

• 发表时间: 2008
• 期刊: Metabolic Syndrome and Related Disorders 6
• 作者: Kaji H;Fukano C;imura Y Takiguchi E;anida K
• 通讯作者: anida K

スプライシング異常が確認できたGitelman症候群の1例

剪接异常Gitelman综合征1例

• 发表时间: 2008
• 作者: 飯田 啓二、野津 寛大、高橋 裕、加治 秀介(5/8)千原 和夫;他
• 通讯作者: 他

The N131S mutation in von Hippel-Lindau gene in a Japanese family with pheochromocytoma and hemangioblastomas.

日本嗜铬细胞瘤和血管母细胞瘤家系中 von Hippel-Lindau 基因的 N131S 突变。

• 发表时间: 2006
• 期刊: Endocrine Journal 53
• 作者: Imanaka M;Iida K;Takahashi K;Tsuji K;Nishikawa;Fukaoka H;Takeno R;Takahashi Y;Okimura Y;Kaji H;Chihara K
• 通讯作者: Chihara K

Hormone replacement therapy and vascular risk disorders in adult hypopituitarism

• DOI: 10.1507/endocrj.k06-129
• 发表时间: 2007-04-01
• 期刊: ENDOCRINE JOURNAL
• 影响因子: 2
• 作者: Kaji, Hidesuke;Iida, Keiji;Chihara, Kazauo
• 通讯作者: Chihara, Kazauo