Research on hypoglycemia caused by inhibition of orexin expression

抑制食欲素表达引起低血糖的研究

• 批准号: 18590991
• 负责人: KUWAJIMA Masamichi
• 金额: $ 2.44万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590991/
• 关键词: JVS mouse; hypoglycemia; death; weaning period; carnitine; milk; cellulose; insulin; レプチン; グルカゴン; インスリン; ラクトース; セルロース

The mechanism of onset of hypoglycemia in patients with carnitine deficiency has yet to be determined. Using mice with systemic carnitine deficiency (JVS mice), we examined this mechanism, focusing on the weaning period (days 1428 postpartum).For normal mice, the survival rate was 100%, and no hypoglycemia was observed at all. Gastric lactose began to decrease on day 17, and callulose increased sharply in amount thereafter.For JVS mice, the survival rate was 77% on day 14 and 28% on day 28. From day 21 on, hypoglycemia was noted. Gastric lactose had disappeared almost completely by day 17, and cellulose was almost undetectable from days 14 to 28. Expression of orexin mRNA in the hypothalamus did not differ between JVS and normal rake on day 14, but was suppressed in JVS mice on days 21 and 28. When JVS mire were fed a carnitine-rich diet, suppression of expression of orexin mRNA in hypothalamus was eliminated, and on day 28 lactose and cellulose were detected in the stomach without hypoglycemia.In conclusion, the suppression of the expression of orexin in the hypothalamus during the weaning period may be involved in the marked anorexia in JVS mice, which eventually leads to death from hypoglycemia.

肉碱缺乏症患者发生低血糖的机制尚未确定。我们使用全身性肉碱缺乏的小鼠（JVS小鼠）来研究这一机制，重点关注断奶期（产后1428天）。对于正常小鼠来说，存活率为100%，并且根本没有观察到低血糖。第17天胃乳糖开始减少，此后胼胝质数量急剧增加。对于JVS小鼠，第14天的存活率为77%，第28天的存活率为28%。从第21天开始，出现低血糖。到第 17 天，胃乳糖几乎完全消失，从第 14 天到第 28 天，纤维素几乎检测不到。第 14 天，JVS 小鼠和正常耙子之间下丘脑中食欲素 mRNA 的表达没有差异，但在第 21 天和第 21 天，JVS 小鼠中食欲素 mRNA 的表达受到抑制。 28. 当 JVS mire 喂食富含肉碱的饮食时，下丘脑中食欲素 mRNA 表达的抑制被消除，并且在第 28 天胃中检测到乳糖和纤维素，但未出现低血糖。综上所述，断奶期间下丘脑食欲素表达的抑制可能与JVS小鼠明显厌食，最终导致低血糖死亡有关。

项目成果

Reduced Carnitine Level Causes Death from Hypoglycemia:Possible Involvement of Suppression of Hypothalamic Orexin Expression During Weaping Period

肉碱水平降低导致低血糖死亡：可能与戒断期下丘脑食欲素表达抑制有关

• 发表时间: 2007
• 期刊: Endocrine Journal 54
• 作者: M. Kuwajima;et. al.
• 通讯作者: et. al.

Impaired long-chain fatty acid metabolism in mitochondria causes brain vascular invasion by a non-neurotropic epidemic influenza A virus in the newborn/suckling period

线粒体长链脂肪酸代谢受损导致新生儿/哺乳期非神经营养性流行性甲型流感病毒侵入脑血管

• 期刊: Mol Cell Biochem (In press)
• 作者: Katsumi;Yamada.;et. al.;D.Yao
• 通讯作者: D.Yao