The investigation of gastric mucosal epithelial cell proliferative factor in Helicobacter pylori

幽门螺杆菌胃粘膜上皮细胞增殖因子的研究

• 批准号: 18590673
• 负责人: YAMAZAKI Yukinao
• 金额: $ 2.34万
• 依托单位: University of Fukui
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590673/
• 关键词: H-pylori; caz PAI; Proliferative Factor; H.pylori; cagPAI; 感染症

[Aim]In this research, we focused the pathogenic factor CagA, encoded in cag pathogenicity island (cag PAI) of Helicobacter pylori (H. pylori) and CagY, one of the proteins composed the type IV secretion system. The aim of this study was to investigate the gastric mucosal epithelial cell proliferative factor in H. pylori using the wild type, cagA knock-out mutant (ΔcagA) and cagY knock-out one (ΔcagY).[Plans] 1. The comparative evaluation of the changes of AGS cell form by the wild type, ΔcagA and ΔcagY infections.2. The evaluation of the molecular weight of CagA, in the wild type, ΔcagA and ΔcagY using the immunohistochemistry.3. The evaluation of the intracellular existence and the location of CagY using the vector with the fluorescence method.[Results] AGS cells infected ΔcagY did not show the hummingbird phenomenon, which was markedly demonstrated at the ones infected the wild type. However, AGS cells infected ΔcagA mildly showed the hummingbird phenomenon. It seemed that CagY play an important role to induce the hummingbird phenomenon at the cells infected H. pylori. Moreover, the immunohistochemistry to the CagA protein existed in the H. pylori demonstrated that the molecular weight of CagA in the ΔcagY was different from that in the wild type and ΔcagA. It suggested that the molecular weight of CagA depended on the CagY protein. These results suggested that CagY, interacted with pathogenic factor CagA might be important factor in relation to the pathogenicity of H. pylori. On the other hand, to evaluate the intracellular existence and the location of CagY using the vector with fluorescence method was failed regrettably.[Conclusion] It seemed that CagY, interacted with pathogenic factor CagA, was likely to play an important role in relation to the pathogenicity of H. pylori.

[目的]本研究重点关注幽门螺杆菌(H. pylori)的cag致病岛(cag PAI)编码的致病因子CagA和组成IV型分泌系统的蛋白质之一CagY。目的是利用野生型、cagA 敲除突变体 (ΔcagA) 和 H. pylori 来研究胃粘膜上皮细胞增殖因子cagY敲除一号(ΔcagY)。[方案] 1.野生型、ΔcagA和ΔcagY感染后AGS细胞形态变化的比较评价。2.野生型中CagA的分子量评价。免疫组织化学法检测ΔcagA和ΔcagY。3．荧光法评价CagY在细胞内的存在和定位。[结果]感染ΔcagY的AGS细胞没有表现出蜂鸟现象，而感染ΔcagA的AGS细胞则轻微地表现出蜂鸟现象，看来CagY在诱导蜂鸟现象中发挥了重要作用。此外，对H. pylori中存在的CagA蛋白进行免疫组化分析，发现ΔcagY中CagA的分子量为与野生型和ΔcagA不同，这表明CagA的分子量取决于CagY蛋白。这些结果表明CagY与致病因子CagA的相互作用可能是与幽门螺杆菌致病性相关的重要因素。另一方面，利用荧光法载体评估CagY在细胞内的存在和位置却遗憾地失败了。[结论]CagY似乎与致病因子存在相互作用。 CagA 可能在幽门螺杆菌的致病性中发挥重要作用。

项目成果

Low Serum Pepsinogen I/II Ratio Induces an Increase in Body Mass Index after Eradication of H pylori

根除幽门螺杆菌后，低血清胃蛋白酶原 I/II 比率会导致体重指数增加

• 发表时间: 2006
• 作者: Suto;H.;Matsunaga;S.;Masaki;R.;Inagaki;T.;Satomi;S.;Okuda;T.;Muramatsu;A.;Lee;S.;Ito;Y.;Yamazaki;Y.;Kuriyama;M.;Kato;T.;Azuma;T
• 通讯作者: T

Relationship between the diversity of the cagA gene of Helicobacter pylori and gastric cancer in Okinawa, Japan

• DOI: 10.1007/s00535-006-1838-6
• 发表时间: 2006-06-01
• 期刊: JOURNAL OF GASTROENTEROLOGY
• 影响因子: 6.3
• 作者: Satomi, Satoko;Yamakawa, Akiyo;Azuma, Takeshi
• 通讯作者: Azuma, Takeshi

Low serum pepsinogen I/II ratio induces an increase in body mass index after eradication of H. pylori.

根除幽门螺杆菌后，低血清胃蛋白酶原 I/II 比值会导致体重指数增加。

• 发表时间: 2006
• 作者: Suto H.;Matsunaga S.;Masaki R.;Inagaki T.;Satomi S.;Okuda T.;Muramatsu A.;Lee S.;Ito Y.;Yamazaki Y.;Kuriyama M.;Kato T.;Azuma T.
• 通讯作者: Azuma T.

Novel Diversity Site of Helicobacter pylori VacA is Critical for Cytotoxin Activity and Clinical Outcome

幽门螺杆菌 VacA 的新多样性位点对于细胞毒素活性和临床结果至关重要

• 发表时间: 2007
• 作者: Inagaki;T.;Ito;Y.;Matsunaga;S.;Masaki;R.;Ohtani;M.;Lee;S.;Suto;H.;Kuriyama;M.;Keida;Y.;Azuma;T.;Wada;A.;Hirayama;T.;Yamazaki;Y
• 通讯作者: Y

H. pylori除菌後の長期経過による上腹部症状の変化,「特集Helicobacter pylori感染と機能性dyspepsia(FD)」

幽门螺杆菌根除后长期上腹部症状的变化，《幽门螺杆菌感染与功能性消化不良（FD）特辑》

• 发表时间: 2008
• 期刊: Journal of Helicobacter Research 12(3)
• 作者: 山崎幸直;吉田郁美;松田秀岳;大野 崇;真崎竜邦;里見聡子;大谷昌弘;李 相植;須藤弘之;伊藤義幸;東 健
• 通讯作者: 東 健