Characterization of teleost hematopoietic stem cells

硬骨鱼造血干细胞的表征

• 批准号: 18580193
• 负责人: MORITOMO Tadaaki
• 金额: $ 2.48万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18580193/
• 关键词: Hematopoietic stem cells; Side population cells; Ginbuna; Fish; ABCG2; Zebrafish; 可塑; 再生; 血液; 腎臓

In teleosts, kidney is the major hematopoietic organ in which all lines of hematopoietic differentiation are observed including erythropoiesis, granulopoiesis, and lymphopoiesis. However, little information was available about the hematopoietic stem or progenitor cells in the kidney. In the present studies, we demonstrated the presence of hematopoietic stem cells (HSCs) in kidney hematopoietic tissues using the transplantation system of ginbuna (Carassius auratus langsdorfii) and ginbuna-gold fish hybrids.Kidney hematopoietic cells were obtained from ginbuna (triploid fish) and transplanted into ginbuna-gold fish hybrids (tetraploid fish). We found donor derived triploid blood cells including erythrocytes, thrombocytes, granulocytes and lymphocytes in the recipient for more than 18 months. These results indicate that long-term self-renewal and multipotent HSCs are present in the donor kidney.We also isolated HSCs from ginbuna and zebrafish (Danio rerio) kidney using side population (SP) analysis on the basis of Hoechst dye exclusion. The HSC activity of ginbuna SP cells were evaluated by their potential for long-term repopulation and multilineage differentiation in vivo.

在硬骨鱼中，肾脏是主要的造血器官，在其中观察到所有造血分化系，包括红细胞生成、粒细胞生成和淋巴细胞生成。然而，关于肾脏中造血干细胞或祖细胞的信息很少。在本研究中，我们利用银鱼（Carassius auratus langsdorfii）和银鱼-金鱼杂交体的移植系统证明了肾脏造血组织中存在造血干细胞（HSC）。肾脏造血细胞是从银鱼（三倍体鱼）和金鱼杂交中获得的。移植到银布纳-金鱼杂交体（四倍体鱼）中。我们在受者体内发现了来自供体的三倍体血细胞，包括红细胞、血小板、粒细胞和淋巴细胞，且存在时间超过 18 个月。这些结果表明供体肾脏中存在长期自我更新和多能的HSC。我们还在Hoechst染料排除的基础上使用侧群（SP）分析从银布纳和斑马鱼（Danio rerio）肾脏中分离了HSC。通过体内长期再增殖和多谱系分化的潜力来评估 ginbuna SP 细胞的 HSC 活性。

项目成果

Characterization and looalization of side population(SP)cells in zeb rafish kidney hematopoietic tissue

斑马鱼肾脏造血组织中侧群（SP）细胞的表征和定位

• 发表时间: 2008
• 期刊: Blood 111
• 作者: I. Kobayashi;et. al.
• 通讯作者: et. al.

Cell culture of carp hematopoietic cells ; effect of stromal cells

鲤鱼造血细胞的细胞培养；

• 发表时间: 2007
• 作者: T.;Moritomo;et. al.
• 通讯作者: et. al.

Characterization and localization of side population (SP) cells in zebrafish kidney hematopoietic tissue

• DOI: 10.1182/blood-2007-08-104299
• 发表时间: 2008-02-01
• 期刊: BLOOD
• 影响因子: 20.3
• 作者: Kobayashi, Isao;Saito, Kazuyuki;Nakanishi, Teruyuki
• 通讯作者: Nakanishi, Teruyuki

Demonstration of hematopoietic stem cells in ginbuna carp (Carassius auratus langsdora kidney

银花鲤（Carassius auratus langsdora肾）造血干细胞的示范

• 发表时间: 2006
• 期刊: Developmental ＆ Comparative Immunology 30・1
• 作者: Isao Kobayashi;Mayuko Sekiya;Tadaaki Moritomo;Mituru Ototake;Teruyuki Nakanishi
• 通讯作者: Teruyuki Nakanishi

コイ造血細胞の培養〜T細胞の増殖〜

鲤鱼造血细胞培养～T细胞增殖～

• 发表时间: 2008
• 作者: Isao Kobayashi;Mayuko Sekiya;Tadaaki Moritomo;Mituru Ototake;Teruyuki Nakanishi;片倉 文彦
• 通讯作者: 片倉 文彦