Studies on the properties of toxins containedin boxfish as the causative agents in food poisonings

箱鲀所含毒素作为食物中毒致病因子的特性研究

• 批准号: 18580204
• 负责人: ASAKAWA Manabu
• 金额: $ 2.47万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18580204/
• 关键词: boxfish; Ostreopsis siamensis; dinoflagellate; myoglobinuria; palytoxin; delayed haemolytic activity; parrotfish; LC; MS; g-ストロファンチン; ESM培地; シガテラ毒

The purpose of this is to daffy the properties of the toxins contained in boxfish Ostracion cubicus as the causative agents in food poisonings due to ingestion of them. The toxins as the causative agents was isolated from the boxfish and the specimens of dinoflagellate Ostreopsis siamensis apossible origin of their toxins using some chromatography. After checking their toxicities of them using MY male mice, their chemical properties was examined and cleared using mainly LC/MS. The crude toxin from the box fish O.cubicus and O.siamensis was extracted, and was tasted. The strains of O.siamensis isolated in June, 2005, Nagasaki Prefecture showed toxicity to mice. The mice injected with 1-butanol layer from them showed the common symptoms of convulsion, drowsiness and collapse, and died within 48 h. Toxins isolated showed delayed haemolytic activity with mouse and human erythrocytes, which was inhibited by ouabain. After being injected with toxins, the serum creatine phosphokinase levels of mice were found to be elevated. By LC/MS analysis, the toxin content of this strain of O.siamensis contain was estimated 1.7 pg/cell as PTX.From these results, it can be strongly postulated that the dinoflagellate O.siamensis is the origin of palytoxin like substance which is sequestered by boxfish through food web.

这样做的目的是为了弄清箱鲀中所含毒素的特性，这些毒素是因摄入这些毒素而引起食物中毒的病原体。使用某些色谱法从箱鲀和暹罗骨鞭毛虫标本中分离出作为病原体的毒素，这可能是其毒素的来源。使用 MY 雄性小鼠检查它们的毒性后，主要使用 LC/MS 检查并清除它们的化学性质。提取箱鱼和暹罗箱鱼的粗毒素，并进行品尝。 2005年6月，长崎县分离出的暹罗蚊菌株对小鼠表现出毒性。注射1-丁醇层的小鼠表现出抽搐、嗜睡和虚脱等常见症状，并在48小时内死亡。分离出的毒素对小鼠和人红细胞表现出延迟的溶血活性，而哇巴因可抑制该活性。注射毒素后，发现小鼠血清肌酸磷酸激酶水平升高。通过LC/MS分析，该菌株所含的毒素含量以PTX计估计为1.7 pg/细胞。从这些结果可以强烈推测甲藻是海藻毒素样物质的来源。被箱鲀通过食物网隔离。

项目成果

セブ島オウギガニ科Demania属カニの毒性について

关于宿务岛 Demania 科 Demania 属螃蟹的毒性

• 发表时间: 2007
• 作者: Manabu;Asakawa;浅川 学
• 通讯作者: 浅川 学

Accumulation of tetrodotoxin (TTX) in Pseudocal igus fugu, a parasitic copepod from panther Takifugu pardalis but without vertical transmission - using an immunoenzymatic technique

河豚毒素 (TTX) 在拟东方鲀（一种来自美洲豹 Takifugu pardalis 的寄生桡足类）中的积累，但没有垂直传播 - 使用免疫酶技术

• 发表时间: 2006
• 期刊: Toxicon 48
• 作者: Katsutoshi;Ito;Koichi Ikeda
• 通讯作者: Koichi Ikeda

Analysis method of palytoxin using high performance liquid chromatography

海藻毒素的高效液相色谱分析方法

• 发表时间: 2007
• 期刊: Bull.Shikoku Univ. (B) 24
• 作者: Takefumi;Sagara
• 通讯作者: Sagara

Isolation and characterization of bacteria from the copepod Pseudocaligus fugu ectoparasitic on the panther puffer Takifugu pardalis with the emphasis on TTX

• DOI: 10.1016/j.toxicon.2007.06.021
• 发表时间: 2007-11-01
• 期刊: TOXICON
• 影响因子: 2.8
• 作者: Maran, B. A. Venmathi;Iwamoto, Emi;Boxshall, Geoffrey A.
• 通讯作者: Boxshall, Geoffrey A.

Toxicity of Xanthid crab from Sebu Island, Philippines

菲律宾塞布岛的 Xanthid 蟹的毒性

• 发表时间: 2007
• 作者: Manabu;Asakawa
• 通讯作者: Asakawa