Study on the genetic information carried in DNA conformation and physical properties

DNA构象和物理性质中携带的遗传信息的研究

基本信息

批准号：
18570165
负责人：
OHYAMA Takashi
金额：
$ 2.53万
依托单位：
Waseda University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18570165/
关键词：
genetic information DNA conformation genome Physical properties of DNA chromatin gene expression

项目摘要

The T20 is a synthetic 180 by bent DNA fragment that mimics a part of left-handed supercoils. T20 can activate transcription by organizing local chromatin structure in HeLa cells. This study investigated whether T20 could activate transcription in the genome of mouse ES cells. T20 was found to activate the adjacent promoter even in the ES cells. Furthermore, the function of T20 was maintained even when the cells were differentiated into hepatocytes. We also investigated the locus of the reporter by using lacO/lacI-GFP transgene detection system. We constructed a tandem-repeat of the lac operator sequences(64 repeats), ligated it to the reporter construct, and integrated the resulting construct into the genome of ES cells. The T20-containing reporter was found located in the periphery of the nucleus.II. Genetic information carried in physical properties of DNAWe made a program that can calculate the flexibility profile of a given DNA. Using it, we investigated the flexibility profiles of all of the recombination hot spots of Saccharomyces cerevisiae chromosome 3. These sites were indicated to be slightly more flexible when compared to the average flexibility of the yeast genome. In addition, we found that the exons are more flexible than the introns. This difference may function as a signal for some proteins to distinguish these two regions.

T20是弯曲DNA片段的合成180，模仿了左手超级锅的一部分。 T20可以通过组织HeLa细胞中的局部染色质结构来激活转录。这项研究研究了T20是否可以激活小鼠ES细胞基因组中的转录。发现T20即使在ES细胞中也可以激活相邻的启动子。此外，即使细胞分化为肝细胞，也保持T20的功能。我们还使用LACO/LACI-GFP转基因检测系统研究了记者的轨迹。我们构建了LAC操作员序列的串联重复（64个重复），将其连接到报告基因构建体，并将所得构建体集成到ES细胞的基因组中。发现含T20的记者位于核的外围。 DNAWE物理特性中携带的遗传信息制作了一个可以计算给定DNA的柔韧性曲线的程序。使用它，我们研究了酿酒酵母3染色体的所有重组热点的柔韧性曲线。与酵母基因组的平均灵活性相比，这些位点的灵活性略有灵活性。此外，我们发现外显子比内含子更灵活。这种差异可能是某些蛋白质区分这两个区域的信号。