Molecular studies on incubation behavior and the application

孵化行为的分子研究及应用

基本信息

批准号：
07044190
负责人：
SHIMADA Kiyoshi
金额：
$ 8.58万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07044190/
关键词：
prolactin incubation behavior chicken mRNA prolactin receptor pituitary hypothalamus PRL mRNA VIP Receptor GnRH antisense PRLmRNA GHmRNA VIP GRF cyclic AMP PRL-Rc recombinant PRL

项目摘要

Now commercial White Leghorn strain chicken dose not express incubation behavior, but Japanese native strain chicken and turkey hens still express incubation behavior. Therefore, in these birds, the incubation behavior lowers productivity of meat and egg laying, causing a lot of economic loss in poulty industries. Therefore, the poupose of present study is to reveal molecular mechanism of the incubation behavior and to establish the application for the removal of incubation behavior of these birds.Turkey and Gifu-jidori hens still express incubation behavior. During the reproductive cycle in these birds, levels of plasma prolactin (PRL) and pituitary PRL mRNA increased during the incubation period but these levels were low in the laying period. These results suggest that PRL may regulate induction and maintaining of incubation behavior.Using perifusion culture of the pituitary and cell culture of pituitary cells, we demonstrated that VIP induces PRL synthesis and release in the pituita … More ry gland via cAMP cascade. We cloned chicken VIP receptor mRNA.There were no significant difference in the distribution of brain PRL receptor mRNA between chabohen displaying expression of incubation behavior and White Leghorn hens without expression of incubation behavior. In addition, during reproductive cycle in chabo hen the levels of brain PRL receptor mRNA did not change.Effects of remobal of newly hatched chicks on incubation behavior were studied in the chabo hens. Those hens whose chicks were removed from the nest for 24 hr displayd again incubation behavior but those hens whose chicks were removed from the nest for 72 hr did not show incubation behavior again. The plasma levels of PRL decreased in all hens whose chicks were removed from the nest for 24 and 72hr. This results suggested that other factor may regulate the maintain of incubation behavior.The administrations of VIP antibody into turkey and chicken hens markedly reduced plasma levels of PRL and inhibited incubation behavior. Less

目前商品化的白来航品系鸡不表现出孵化行为，但日本本土品系鸡和火鸡母鸡仍表现出孵化行为，因此，在这些禽类中，孵化行为降低了产肉和产蛋的生产率，给家禽造成很大的经济损失。因此，本研究的目的是揭示孵化行为的分子机制，并建立消除这些鸟类孵化行为的应用程序。火鸡和岐阜地鸡仍然表现出孵化行为。在这些鸟类的繁殖周期中，血浆催乳素 (PRL) 和垂体 PRL mRNA 的水平在孵化期间增加，但在产蛋期间这些水平较低，这些结果表明 PRL 可能调节孵化行为的诱导和维持。通过垂体灌注培养和垂体细胞细胞培养，我们证明了 VIP 通过 cAMP 级联诱导垂体中 PRL 的合成和释放。表达孵化行为的查博母鸡与不表达孵化行为的白来亨鸡脑部PRL受体mRNA分布无显着性差异。此外，在繁殖周期中查博母鸡脑部PRL受体mRNA水平没有变化。在 Chabo 母鸡中研究了移走新孵出的雏鸡对孵化行为的影响，那些将雏鸡移出巢穴 24 小时的母鸡再次表现出孵化行为。血浆行为，但将雏鸡移出巢穴 72 小时的母鸡没有再次表现出孵化行为。将雏鸡移出巢穴 24 小时和 72 小时的所有母鸡的 PRL 水平均下降。这一结果表明，其他因素可能会调节。维持孵化行为。给火鸡和母鸡注射 VIP 抗体可显着降低血浆 PRL 水平并抑制孵化行为。