Integrated and Fictionalized Nanoparticles in 3-dimentional nanospase

三维纳米空间中的集成和虚构纳米粒子

基本信息

批准号：
18510099
负责人：
TOMINAGA Masato
金额：
$ 2.64万
依托单位：
Kumamoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

Carbon nanotubes(CNTs)was synthesized on a platinum plate(99.98 %, 20×20×1 mm)by the chemical vapor deposition(CVD)method using iron nanoparticles derived from ferritin molecules. The synthesized CNTs were characterized by Raman spectroscopy and TEM. Two Raman shift peaks corresponding to G-(ca. 1580 cm^<-1>)and D-bands(ca. 1350 cm^<-1>)were observed on the platinum electrode surface after the synthesis of the CNTs, which indicated that CNTs were present on the electrode surface. The diameters of individual CNTs were evaluated to be ca. 5 to 10 nm, which could be classified as multi-walled CNTs(MWCNTs).The MWCNTs synthesized on the Pt plate (MWCNTs/Pt)electrode were immediately immersed into solutions of glucose oxidase (GOX)to immobilize these enzymes onto the MWCNTs/Pt electrode surfaces. After the GOX was immobilized onto the MWCNTs/Pt electrode, a well-defined catalytic oxidation current was increased from ca. -0.45 V (vs. Ag/AgCl/sat'd KCl), which was close to the redox potential of flavin adenine dinucleotide (FAD)as a prosthetic group of GOX under physiological pH values. Also, fructose dehydrogenase (FDH)immobilized MWCNTs/Pt electrode showed a well-defined catalytic oxidation current based on FDH was observed from ca. -0.15 V (vs. Ag/AgCl/sat'd KCl), which was close to the redox potential of heme c as a prosthetic group of FDH. From an analysis of a plot of the catalytic current vs. substrate, the calibration range for the fructose concentration was up to ca. 40μmol dm^<-3>, and the apparent Michaelis-Menten constant was evaluated to be 11 (±)mmol dm^<-3>Further investigations concerning both the interactions between enzymes and MWCNTs and direct heterogeneous electron transfer reactions are underway to develop sensor applications and glucose-oxygen biofuel cells.

使用源自铁蛋白分子的铁纳米颗粒，通过化学蒸气沉积（CVD）方法在铂板（99.98％，20×20×1 mm）上合成碳纳米管（CNT）。合成的CNT以拉曼光谱和TEM为特征。在CNT合成后，在铂电极表面上观察到了与G-（Ca。1580cm^<-1>）相对应的两个拉曼移位峰（Ca。1580cm^<-1>>>）（Ca。1350cm^<-1>），这表明CNT表明CNT在电极表面显示。评估单个CNT的直径为CA。 5至10 nm，可以分类为多壁CNT（MWCNTS）。在PT板（MWCNT/PT）电极上合成的MWCNTS立即浸入氧化葡萄糖溶液中，以将这些酶固定在MWCNTS/PT Electrode电极上。将GOX固定到MWCNT/PT电极上后，从Ca中增加了明确的催化氧化电流。 -0.45 V（vs. AG/AGCL/SAT'D KCl），它接近黄素腺嘌呤二核苷酸（FAD）作为物理pH值下的GOX假体的氧化还原电位。同样，固定的MWCNT/PT电极果糖脱氢酶（FDH）显示出基于FDH的明确定义的催化氧化电流。 -0.15 V（vs. AG/AGCL/SAT'D KCL），它接近血红素C作为FDH假肢的氧化还原电位。从对催化电流与底物的图的分析中，果糖浓度的校准范围高于Ca。 40μmolDM^<-3>评估明显的Michaelis-Menten常数为11（±）MMOL DM^<-3>有关酶与MWCNT之间的相互作用以及直接异构电子转移反应的进一步研究，以开发传感器应用和glucose-Oxy-oxy-oxy-oxy-oxy-oxy-oxy-oxy-oxy-oxy-oxy-oxy-oxy-oxy-offioffiel细胞。