Integrated and Fictionalized Nanoparticles in 3-dimentional nanospase

三维纳米空间中的集成和虚构纳米粒子

基本信息

批准号：
18510099
负责人：
TOMINAGA Masato
金额：
$ 2.64万
依托单位：
Kumamoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

Carbon nanotubes(CNTs)was synthesized on a platinum plate(99.98 %, 20×20×1 mm)by the chemical vapor deposition(CVD)method using iron nanoparticles derived from ferritin molecules. The synthesized CNTs were characterized by Raman spectroscopy and TEM. Two Raman shift peaks corresponding to G-(ca. 1580 cm^<-1>)and D-bands(ca. 1350 cm^<-1>)were observed on the platinum electrode surface after the synthesis of the CNTs, which indicated that CNTs were present on the electrode surface. The diameters of individual CNTs were evaluated to be ca. 5 to 10 nm, which could be classified as multi-walled CNTs(MWCNTs).The MWCNTs synthesized on the Pt plate (MWCNTs/Pt)electrode were immediately immersed into solutions of glucose oxidase (GOX)to immobilize these enzymes onto the MWCNTs/Pt electrode surfaces. After the GOX was immobilized onto the MWCNTs/Pt electrode, a well-defined catalytic oxidation current was increased from ca. -0.45 V (vs. Ag/AgCl/sat'd KCl), which was close to the redox potential of flavin adenine dinucleotide (FAD)as a prosthetic group of GOX under physiological pH values. Also, fructose dehydrogenase (FDH)immobilized MWCNTs/Pt electrode showed a well-defined catalytic oxidation current based on FDH was observed from ca. -0.15 V (vs. Ag/AgCl/sat'd KCl), which was close to the redox potential of heme c as a prosthetic group of FDH. From an analysis of a plot of the catalytic current vs. substrate, the calibration range for the fructose concentration was up to ca. 40μmol dm^<-3>, and the apparent Michaelis-Menten constant was evaluated to be 11 (±)mmol dm^<-3>Further investigations concerning both the interactions between enzymes and MWCNTs and direct heterogeneous electron transfer reactions are underway to develop sensor applications and glucose-oxygen biofuel cells.

利用铁蛋白分子衍生的铁纳米粒子，通过化学气相沉积（CVD）方法在铂板上（99.98％，20×20×1mm）合成碳纳米管（CNT），并通过拉曼光谱和TEM对合成的CNT进行了表征。两个拉曼位移峰对应于 G-(约 1580 cm^<-1>) 和 D-带(约 1580 cm^-1>)。合成CNT后，在铂电极表面观察到了1350cm^-1)，这表明电极表面上存在CNT。单个CNT的直径被评估为约5至10nm。可归类为多壁碳纳米管（MWCNTs）。在铂板（MWCNTs/Pt）电极上合成的多壁碳纳米管立即浸入溶液中葡萄糖氧化酶（GOX）将这些酶固定到 MWCNTs/Pt 电极表面上。将 GOX 固定到 MWCNTs/Pt 电极上后，明确的催化氧化电流从约 -0.45 V 增加。 AgCl/sat'd KCl)，接近黄素腺嘌呤二核苷酸 (FAD) 作为辅基的氧化还原电位此外，果糖脱氢酶 (FDH) 固定的 MWCNT/Pt 电极显示出基于 FDH 的明确催化氧化电流（相对于 Ag/AgCl/sat'd KCl）。，从催化电流与催化电流图的分析来看，它接近作为 FDH 辅基的血红素 c 的氧化还原电位。底物，果糖浓度的校准范围高达约 40μmol dm^<-3>，表观 Michaelis-Menten 常数估计为 11 (±)mmol dm^<-3> 关于这两种相互作用的进一步研究酶和多壁碳纳米管之间的相互作用以及直接异质电子转移反应正在进行中，以开发传感器应用和葡萄糖氧生物燃料电池。