Effect of mechanical stress on responses of oxidative stress

机械应力对氧化应激反应的影响

• 批准号: 17500415
• 负责人: NOMURA Jun
• 金额: $ 2.31万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17500415/
• 关键词: cell stretching; cDNA microarray; panel of antibody; fibroblast; mechanical stress; MAP kinase; cell adhesion; oxidative stress; メカノストレス; 培養ヒト線維芽細胞; プロテオーム解析; 細胞骨格; シャペロン分子; cDNA array

Oxidative stress have been implicated in a variety of human disorders including cancer and aging. We reported that exposure to mechanical stress increased urinaly 8-OHdG levels. Moreover repetitive exposure of mechanical stress reduced the 8-OHdG level These observations suggested that repetition of mechanical stress induced a tolerance. However, there are no reports about tolerance mechanisms about tolerance of mechanical stress.In this study, we analyzed relationship of responsive mechanisms between mechanical stress and oxidative stress. Mechanical stress is recognized as an important extracellular stimulus that promotes cellular growth and survival, influences metabolic processes (including gene expression), and governs tissue architecture in various cell types. Fibroblast cells were cultured on collagen coated elastic silicon chamber. And then, uni-axial stretch was applied to the cells by a computer-controlled stretching apparatus. Cells were also exposed to an oxidative stress by addition of hydrogen-oxide to the medium. Expression levels of genes and proteins were analyzed by cDNA array nad western blotting methods. We identified several differentially expressed genes and proteins, including the ER stress marker glucose-regulated protein 78 (GRP78) and ERK1. Moreover, knockdown of one of tetraspani web moles induced detachment of cells from a culture apparatus.Therefore, molecules which responsive to the oxidative stress during exposure to the mechanical stress, related to the regulation of protein expression.

氧化应激与包括癌症和衰老在内的多种人类疾病有关。我们报告说，暴露于机械应力增加了尿液尿管8-OHDG水平。此外，机械应力的重复暴露降低了8-OHDG水平，这些观察结果表明，机械应力的重复会引起公差。但是，没有关于机械应力耐受性的耐受性机制的报道。在这项研究中，我们分析了机械应力与氧化应激之间的响应机制的关系。机械应力被认为是一种重要的细胞外刺激，可促进细胞生长和存活，影响代谢过程（包括基因表达），并控制各种细胞类型的组织结构。成纤维细胞在胶原蛋白涂层的弹性硅腔室中培养。然后，通过计算机控制的拉伸设备将单轴拉伸应用于细胞。还通过将氢氧化添加到培养基中，将细胞暴露于氧化应激。通过cDNA阵列NAD Western印迹方法分析基因和蛋白质的表达水平。我们确定了几种差异表达的基因和蛋白质，包括葡萄糖调节的蛋白78（GRP78）和ERK1。此外，敲低四翼虫网络摩尔人会诱导细胞与培养设备的脱离。因此，与蛋白质表达有关的机械应激期间氧化应激的反应的分子。

项目成果

Modulation of mutability in UV-irradiated human RSb cells by serum obtained from parabolic flight volunteers

抛物线飞行志愿者血清对紫外线照射的人类 RSb 细胞突变性的调节

• 发表时间: 2007
• 期刊: J. Int. Soc. Life Info. Sci. 25
• 作者: Chi;X.;Takahashi;S.;Nomura;J.;Sugaya;S.;Ishimura;Y.;Zhai,L.;Tong;X.;Kita;K.;Suzuki;N.
• 通讯作者: N.

Enhanced expression of Transsferrin receptor confers UV-resistance in human and monkey cells

转铁蛋白受体表达增强赋予人和猴细胞抗紫外线能力

• 发表时间: 2005
• 期刊: Journal of Radiation Research 46
• 作者: Chen;Z-H.
• 通讯作者: Z-H.

周期的伸展後のケロイド由来皮膚線維芽細胞で誘発される遺伝子の解析

循环拉伸后瘢痕疙瘩源性真皮成纤维细胞诱导基因分析

• 发表时间: 2006
• 作者: Nomura;J;et. al.;野村 純;金沢 雄一郎
• 通讯作者: 金沢 雄一郎

細胞伸展により誘導される遺伝子のマイクロアレイ解析

细胞扩散诱导基因的微阵列分析

• 发表时间: 2005
• 作者: Kanazawa;Y;et. al.;金沢 雄一郎
• 通讯作者: 金沢 雄一郎

Modulation of mutability in UV-irradiated human RSb cells by serum obtained parabolic flight volunteers

抛物线飞行志愿者血清对紫外线照射的人类 RSb 细胞突变性的调节

• 期刊: Journal of International Society of Life Information Science (In press)
• 作者: Chen;Z-H.;Xio-Jun Chi
• 通讯作者: Xio-Jun Chi