Investigation on Kampo Drug Resources in Asia and Analysis on Diversity ofMedicinal Plants

亚洲汉方药资源调查及药用植物多样性分析

基本信息

批准号：
17406004
负责人：
KOMATSU Katsuko
金额：
$ 4.74万
依托单位：
University of Toyama
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17406004/
关键词：
medicinal plants Chinese crude drug molecular systematics quality evaluation dementia hepatitis C virus international exchange of researcher China : Mongolia : Thailand 薬用植物抗炎症作用痴呆中国:モンゴル:タ

项目摘要

1. Genetic and chemical diversity(1) Rheum palmatum, R. tanguticum and R. officinale presented 22, 4 and 3 genotypes in matK gene, respectively and each showed regional specificity. Quality evaluation on 28 compounds revealed R. palmatum belonged to subclade I was of high quality.(2) DNA microarray for authentication of ginseng drugs has been developed using genetic data of Panax plants. Analysis of ginsenosides by LC-FTICR-MS provided different mass chromatographic profiles for each species.(3) Based on common sequences of 15 genotypes of Eleutherococcus senticosus in trnK gene, an identification method applying PCR-RFLP was developed. Production areas of rhizomes containing over 0.05% of each eleutherosides B and E were determined(4) Regards to astragalosides and isoflavonoids contents, Astragali Radix cultivated in Inner Mongolia were with high quality as wild ones in Shanxi Prov. Comprehensive analysis of chemical constituents and multivariate data analysis showed the difference be … More tween Mongolian and Chinese Astragali Radix.(5) 8 Ephedra species from Mongolia presented 14 genotypes in 18S rRNA-trnK gene sequences. In southwestern Mongolia, possible hybrids-derived populations were observed. 6 species contained more than 1.5% of ephedrine alkaloids.(6) 7 types of volatile constituents' compositions were observed in "Gajutsu." Gajutsu derived from Curcuma phaeocaulis and from Japanese C. zedoaria belonged to the same type. Curcuma rhizomes from Thailand presented 9 types of volatile constituents' compositions, which related to genotypes.2. Pharmacological active compounds(1) Eleutherococcus Senticosus Rhizome showed significant effects on axonal and dendritic regenerations and synaptic reconstruction in A8(25-35)-treated rat cortical neurons. Eleutheroside B was identified as one of active components.(2) Through chemomatric analysis on Curcuma phaemcanlis rhizome, furanodienone and curcumenol were determined as active compounds responsible for COX-2 inhibitory activity.(3) In root bark of Tripterygium hypoglaucum which showed inhibitory effect on hepatitis C viral polymerase, demethylzeylasteral was identified as an active compound. Less

1。遗传和化学多样性（1）Rheum Palmatum，R。Tanguticum和R. officinale分别在MATK基因中呈现22、4和3基因型，每个基因型都显示出区域特异性。在28种化合物上的质量评估显示，棕榈杆菌属于Is克莱德I的高质量。（2）使用Panax植物的遗传数据开发了用于人参药物身份验证的DNA微阵列。通过LC-FTICR-MS对人参皂苷的分析为每种物种提供了不同的质量色谱谱。（3）基于TRNK基因中15个基因型Eleutherococcus senticosus的共同序列，开发了一种应用PCR-RFLP的鉴定方法。确定了含有超过0.05％的每种蛋白质B和E 0.05％的根茎的生产区域（4）（4）对黄质剂和异黄酮含量，在蒙古内培养的阿斯特拉加利辐射含量是高质量的，在山西省的野生动物中具有高质量。化学构成和多元数据分析的全面分析表明，蒙古和中国阿斯特拉加利辐射的差异是差异。（5）来自蒙古的8种麻质物种在18S rRNA-TRNK基因序列中呈现了14种基因型。在蒙古西南部，观察到可能由杂种衍生的人群。 6种包含超过1.5％的麻黄碱生物碱。（6）在“ gajutsu”中观察到7种挥发性构成的组成。源自curcuma phaeocaulis和日本Zedoaria属于同一类型的高朱苏（Gajutsu）。来自泰国的姜黄根茎呈现了9种与基因型相关的挥发性构成类型。2。药理学活性化合物（1）Eleutherococus Senticosus Rhizome对A8（25-35）处理的大鼠皮质神经元的轴突和树突再生和突触重建表现出显着影响。（2）通过对Curcuma phaemcanlis根茎，Furanodienone和姜黄素的化学分析确定为活性成分之一。活性化合物。较少的