Development of anti-cancer therapy targeting U7 (C7orf24)

开发针对 U7 (C7orf24) 的抗癌疗法

• 批准号: 17390437
• 负责人: KAGEYAMA Susumu
• 金额: $ 9.98万
• 依托单位: Shiga University of Medical Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17390437/
• 关键词: Proteomics; Cancer-related protein; RNAi; Cancer treatment; RNA干渉

Proteome analysis of bladder cancer with narrow-range pH 2-DE has identified a novel protein on chromosome 7 encoded by ORF 24(C7orf24) as one of the highly expressed proteins in cancer cells. C7orf24 is currently registered in the protein database as a hypothetical protein with unknown function. The homologs of C7orf24 in other animals have also been registered as putative protein genes. Western blot analysis using a mAb against C7orf24 confirmed its higher expression in bladder cancer compared with normal tissue. Several other cancer cell lines were also found to express C7orf24. However, the introduction of C7orf24 into Rat-1 or NIH3T3 cells did not cause malignant transformation. A stable transfectant of. NIH3T3 cells with recombinant retrovirus vector was produced for a growth rate assay, and a higher growth rate was observed in C7orf24-expressing cells compared with the controls. Six kinds of small interfering RNAs (siRNAs) were then produced, and C7orf24-siRNA#5 showed a strong knockdown effect on protein expression and significant antiproliferative effects on cancer cell lines were demonstrated by the MTT assay. Therefore, C7orf24. may have an important role in cancer cell proliferation, and may be an appropriate therapeutic target molecule against cancer.

用窄范围pH 2-DE对膀胱癌的蛋白质组分析已经确定了由ORF 24（C7orf24）编码的7染色体上的新蛋白质，是癌细胞中高度表达的蛋白质之一。 C7ORF24目前在蛋白质数据库中注册为具有未知功能的假设蛋白质。其他动物中C7ORF24的同源物也已注册为假定的蛋白质基因。使用MAB对C7ORF24的MAB分析证实，与正常组织相比，其在膀胱癌中的表达更高。还发现其他几种癌细胞系表达C7orf24。但是，将C7orf24引入大鼠-1或NIH3T3细胞不会引起恶性转化。一个稳定的转染剂。用于生长速率分析产生具有重组逆转录病毒载体的NIH3T3细胞，与对照组相比，在表达C7ORF24的细胞中观察到更高的生长速率。然后产生六种小型干扰RNA（siRNA），C7ORF24-SIRNA＃5对蛋白质表达产生了强烈的敲低作用，MTT分析证明了对癌细胞系的明显抗增殖作用。因此，C7orf24。可能在癌细胞增殖中起重要作用，并且可能是针对癌症的适当治疗靶标分子。

项目成果

A novel tumor-related protein, C7orf24, identified by proteome differential display of bladder urothelial carcinoma

• DOI: 10.1002/prca.200600468
• 发表时间: 2007-02-01
• 期刊: PROTEOMICS CLINICAL APPLICATIONS
• 影响因子: 2
• 作者: Kageyama, Susumu;Iwaki, Hideaki;Yoshiki, Tatsuhiro
• 通讯作者: Yoshiki, Tatsuhiro