The development of tissue engineering procedure for osteogensesis -investigation using plate rich plasma and mesenchymal stem cell-

成骨组织工程程序的开发 - 使用富含平板的血浆和间充质干细胞进行研究 -

基本信息

批准号：
17390416
负责人：
ISHIGURO Naoki
金额：
$ 9.63万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

项目摘要

Distraction osteogenesis (DO) has become a widely used and accepted procedure in patients with limb length discrepancy or large bone defect, but the treatment period is long which results in higher rates of complications. Good bone formation during DO is essential to minimize the treatment time and reduce associated complications. We considered that DO is good model for investigating the biology of osteogensis. Tissue engineering strategy, which consists of three essential components (application of osteogenic cells, osteoinductive factors, and a scaffold), has emerged as a possible alternative to accelerate bone regeneration at the distracted gap.Bone marrow-derived mesenchymal stem cells(BMC)can be directed towards the osteogenic lineage if cultured in the presence of dexamethasone. Osteogenic potential can be maintained even in BMSCs that have been passaged several times in the presence of hrBMP-2. Platelet rich plasma(PRP)contains several osteoinductive growth factors including pla … More telet-derived growth factor, insulin-like growth factor, and transforming growth factors. PRP increased the number of BMC, but did not promote osteogenic potential in cultured BMC cells. Several investigators have reported that the injection of cells into the distracted callus enhanced bone formation However, the injected cells may move to the surrounding tissue, leading to a reduced concentration in the target area. Therefore, the scaffold is important factor. We concluded PRP can be a suitable carrier for cell transplantation because it coagulates immediately by an addition of thrombin and calcium from our studies. The weight bearing and movement may be another factor to develop osteogenesis in DO. We revealed that the weight bearing with distracted leg stimulated bone formation compared to non-weight bearing condition.For the clinical application of BMC based therapy, autologous PRP was used to prevent disease transmission and immunoreactions. The transplantation of BMC and PRP during DO may be regarded as promising procedure. Less

牵引成骨（DO）已成为肢体长度不等或大骨缺损患者广泛使用和接受的手术，但治疗周期长，导致并发症发生率较高，DO期间良好的骨形成对于最大限度地缩短治疗时间至关重要。我们认为 DO 是研究成骨生物学的良好模型，该策略由三个基本组成部分（成骨细胞、骨诱导因子和支架的应用）组成，已成为一种可能的替代方案。加速骨再生如果在地塞米松存在下培养，骨髓来源的间充质干细胞 (BMC) 可以定向向成骨谱系，即使在 hrBMP-2 存在下传代数次的 BMSC 中也可以保持成骨潜力。富血小板血浆（PRP）含有多种骨诱导生长因子，包括血小板源性生长因子、胰岛素样生长因子和增加 PRP 数量的转化生长因子。一些研究人员报告说，将细胞注射到分散的愈伤组织中可以增强骨形成，但注射的细胞可能会移动到周围组织，导致目标区域的浓度降低。因此，我们得出结论，PRP 可以成为细胞移植的合适载体，因为根据我们的研究，它通过添加凝血酶和钙而立即凝固，负重和运动可能是 DO 中成骨的另一个因素。我们透露与非负重条件相比，分心腿的负重刺激了骨形成。对于基于BMC的治疗的临床应用，自体PRP用于预防疾病传播和免疫反应，DO期间BMC和PRP的移植可能被认为是有前途的。程序.更少