The experimental study about liver grafts from Marginal Donor

边缘供肝移植的实验研究

• 批准号: 17390343
• 负责人: SATOMI Susumu
• 金额: $ 9.79万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17390343/
• 关键词: energy status; Pre-cold preservation perfusion,; non-heart-beating donor; biliverdin; edaravone; 過少グラフト肝移植; 肝重量当たり門脈血流量; 類洞内皮障害; 微小循環障害; 門脈下大静脈シャント

Background. For a successful liver transplantation (LTx) from non-heart-beating donors (NHBD), it is necessary to clarify the mechanism of the liver grafts injuries. In this study we investigated the effects of pre-cold preservation perfusion on the grafts for the purpose of the maintenance of the energy status and microcirculation. We also investigated the effects of the antioxidant, biliverdin, and free radical scavenger, edaravone on liver grafts to reduce inflammatory reaction.Methods. Male Wistar rats were used. Livers were retrieved, preserved in UW solution, and perfused for ) 60 minutes with oxygenated Krebs-Henseleit solution. Rats were allocated to six groups as follows (n=5) : i)Control group; no warm ischemia (WI) and cold preservation, ii)HBD group; no WI with cold preservation for 6 hours, iii)NHBD group; with 30 minutes of WI and cold preservation, iv)PRE group ; with WI, 30 minutes pre-cold preservation perfusion after cardiac arrest, and cold preservation, v)BV group; addition of biliverdin to pre-cold preservation perfusion, vi)ED group; addition of biliverdin to pre-cold preservation perfusion. Portal flow and bile production during reperfusion, AST and TNF-α in perfusate, energy charge (EC) and ATP level in the tissue, and histological findings were investigated.Results. Portal flow volume in the PRE, ED, and BV groups were higher than in NHBD group. Bile production in the PRE, ED, and BV groups were also higher than in the NHBD group. Increase of AST and TNF-a was reduced in the BV group. EC and ATP level of the BV group after reperfusion were higher than those of the NHBD group.Conclusions. Pre-cold preservation perfusion and addition of edaravone or biliverdin to perfusate improved viability of grafts from NHBD. The results mean that to preserve the energy status and microcirculation of the graft is important for successful LTx from NHBD.

背景。在本研究中，从非心态的供体移植（NHBD）。检索抗氧化剂和RAD肝的作用，在UW溶液中保留了60分钟的液体。 HBD组; iii dece pred to，iii）pre pree to defiperd; iii;在灌注液中，组织学中的AST和TNF-α在组织学发现和组织学发现中。在BV组中，AST和TNF-A的增加。来自NHBD的Uccessful LTX。

项目成果

Excessive portal flow causes graft failure in extremely small-for-size liver transplantation in pigs

猪超小型肝移植中门脉血流过多导致移植失败

• 发表时间: 2005
• 期刊: World Journal of Gastroenterology Vol.11(44)
• 作者: H.S.Wang;S.Satomi;et al.
• 通讯作者: et al.

Excessive Portal Flow Causes Graft Nonfunction in Small Size Liver Transplantation : An Experimental Study in Pigs

门脉血流过多导致小体积肝移植中移植物无功能：猪实验研究

• 发表时间: 2005
• 期刊: Transplantation Proceedings 37
• 作者: H.S.Wang;S.Satomi;et al.
• 通讯作者: et al.

The Effect of Perfusion Prior to Cold Presevation and Addition of Biliverdin on the liver Graft from Non-Heart-Beating Donors.

冷保存前灌注和添加胆绿素对无心跳供体肝移植的影响。

• 发表时间: 2006
• 期刊: Transplantation Proceedings 38
• 作者: Iwane T.;Akamatsu Y.;Narita T.;Nakamura A.;Satomi S.
• 通讯作者: Satomi S.