Construction of artificial symbiotic community using Chlorella-symbiont association and it's application

小球藻-共生体人工共生群落的构建及其应用

基本信息

批准号：
17310042
负责人：
TANAKA Hideo
金额：
$ 10.02万
依托单位：
University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

项目摘要

Chlorella sorokiniana IAM C-212 has long been maintained in slant culture as a mixed strain, representing an associated natural microbial consortium. The consortium was separated and four nonalgal constituents, a fungal strain (Acremonium sp.), and three bacterial strains (Ralstonia pickettii, Sphingomonas sp. DD38, Microbacterium trichotecenolyticum) were isolated and identified. The interaction between Chlorella and its symbionts under photoautotrophic conditions involved both mutualism and commensalisms. Sphingomonas sp. And M. trichotecenolyticum were harbored on the sheath produced by Chlorella, while Acremonium sp. And R. pickettii directly adhered to the Chlorella cell surface by using lectin-like substances. The sheath is composed of sugars, amino acids, vitamins and metals. A novel artificial medium that imitates photoautotroph-heterotroph interaction was developed. With the artificial medium, it was possible to selectively isolate some novel bacterial strains. The sheath is a suitable habitat for several symbiotic microorganisms because it ensures close proximity between the C. sorokiniana and symbionts. The sheath was increased by addition of calcium chloride. The sheath resulted in coflocculation of C. sorokiniana and the associated symbiotic bacteria, thus strengthening the bacterial-Chlorella symbiotic association. An application of this technique was demonstrated by constructing a complex of C. sorokiniana and a propionate-degrading bacterium (PDS1). Although propionate inhibited the growth of axenic C. sorokiniana, the C. sorokiniana-PDS1 complex (CPC) showed good growth in a medium containing a high concentration of propionate. The CPC was also used for simultaneous removal of organic acids, nitrate, ammonia and phosphate.

Chlorella sorokiniana IAM C-212 长期以来作为混合菌株维持在斜面培养中，代表相关的天然微生物群落。分离并鉴定了该聚生体，并分离并鉴定了四种非藻类成分、一种真菌菌株（顶孢霉属）和三种细菌菌株（皮氏拉尔斯顿氏菌、鞘氨醇单胞菌DD38、解毛微杆菌）。在光自养条件下小球藻与其共生体之间的相互作用涉及互利共生和共生。鞘氨醇单胞菌属。 M. trichotecenolyticum 寄生在小球藻产生的鞘上，而 Acremonia sp. 寄生在小球藻产生的鞘上。而R.pickettii则利用凝集素类物质直接粘附在小球藻细胞表面。鞘由糖、氨基酸、维生素和金属组成。开发了一种模仿光合自养生物-异养生物相互作用的新型人工培养基。利用人工培养基，可以选择性地分离一些新的细菌菌株。鞘是多种共生微生物的合适栖息地，因为它确保了 C. sorokiniana 和共生体之间的紧密接触。通过添加氯化钙来增加鞘层。鞘导致 C. sorokiniana 和相关共生细菌共絮凝，从而加强细菌-小球藻共生关联。通过构建 C. sorokiniana 和丙酸降解细菌 (PDS1) 的复合物，证明了该技术的应用。尽管丙酸盐抑制了纯 C. sorokiniana 的生长，但 C. sorokiniana-PDS1 复合物 (CPC) 在含有高浓度丙酸盐的培养基中表现出良好的生长。 CPC 还用于同时去除有机酸、硝酸盐、氨和磷酸盐。