Development of artificial arrayed nose system with drug metabolizing enzymes

药物代谢酶人工阵列鼻系统的开发

• 批准号: 17300058
• 负责人: MITSUBAYASHI Kohji
• 金额: $ 10.42万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17300058/
• 关键词: drug metabolizing enzyme; optical fiber; UV-LED; NADH; ethanol vapor; olfactory system; bio-sniffer; nitrogen compound; ニコチン; 酵素阻害; 免疫計測; 大腸菌; SPR; 蛍光計測; 匂いセンサ; バイオセンサ; トリエチルアミン

A novel artificial arrayed nose system with drug metabolizing system was investigated with following steps,(a) optical fluorescent fiber with alcohol dehydrogenase for ethanol vapor,(b) biochemical sniffer with flavin containing monooxygenase as one of the drug metabolizing enzyme,(c) bio-sniffer chip with screen printed electrodes. The detailed results are described as follows.(a) The fiber optic type biochemical gas-sensor(bio-sniffer) was constructed by using NAD+ dependent dehydrogenase enzyme and exciting light source of UV-LED and portable spectrometer: In this sensor system, the concentration of ethanol vapor could be successfully measured by detecting the fluorescent change in NADH as ADH (alcohol dehydrogenase) enzymatic product by the portable fiber optic fluorometric system. The fluorescent sniffer system could be applied to other sniffers for other smell chemicals (acetaldehyde, formaldehyde, lactate, pyruvate, succinate, etc) using their dehydrogenase enzymes. The thinner … More fiber would be suitable for constructing the arrayed nose system for multi analyte in the smell.(b) Triethylamine (TEA) is a colorless volatile organic compounds with a strong fishy malodor, like ammonia, in the gas phase. A bioelectronic sniffer for triethylamine (TEA) was developed with a flavin-containing monooxygenase type 3 (FMO-3). The TEA biosniffer consisted of a Clarktype dissolved-oxygen electrode and an FMO-3 immobilized membrane. In order to amplify the biosensor output was applied. The FMO-3 biosniffer was used to measure TEA vapor.(c) acetaldehyde is one of the specific malodor substances, and its maximum permitted concentration is defined as 25 ppm by ACGIH. The bio-sniffer device was constructed by coating a carbon and Ag/AgCl electrodes on a filter paper, and immobilizing enzyme (ALDH) to the carbon electrode. The ALDH sniffer was used in the gas phase to measure acetaldehyde vapor with the calibration range from 0.2 to 4.0 ppm with good gas selectivity which was attributed to the substrate specificity of ALDH. Less

具有以下步骤的新型人工阵列系统，具有以下步骤，用于乙醇蒸气酶 - 截筛电极的乙醇sniffer芯片。 -LED和便携式光谱仪：在此传感器系统中，酶促的酶浓度（酒精脱氢酶）的酶促预定仪可以应用于其他气味化学物质（乙醛，甲醛，乳酸，乳酸，等）荧光嗅觉系统使用脱水酶，适用于在气味的无色有机化剂中构建阵列的多种分析物。 。滤纸上的AGCl电极。 。 较少的

项目成果

BREATH ANALYSIS WITH BIO-SNIFFERS(BIOCHEMICAL GAS SENSORS) FOR ETHANOL AND ACETALDEHYDE AFTER DRINKING

使用生物嗅探器（生化气体传感器）对酒后乙醇和乙醛进行呼吸分析

• 发表时间: 2007
• 作者: Hiroyuki Matsunagaa;Hirokazu Saito b;Hiroyuki Kudo b and Kohji Mitsubayashi
• 通讯作者: Hiroyuki Kudo b and Kohji Mitsubayashi

A bio-sniffer stick with FALDH (formaldehyde dehydrogenase) for convenient analysis of gaseous formaldehyde

• DOI: 10.1016/j.snb.2007.07.086
• 发表时间: 2008-03-14
• 期刊: SENSORS AND ACTUATORS B-CHEMICAL
• 影响因子: 8.4
• 作者: Mitsubayashi, Kohji;Nishio, Genki;Marty, Jean-Louis
• 通讯作者: Marty, Jean-Louis

An NADH fluorometric biosensor for ethanol measurement

用于乙醇测量的 NADH 荧光生物传感器

• 发表时间: 2007
• 作者: Kohji MITSUBAYASHI;et. al.
• 通讯作者: et. al.

BIOELECTRONIC SNIFFERS FOR FORMALDEHYDE IN THE GAS PHASE

气相甲醛生物电子嗅探器

• 发表时间: 2005
• 期刊: Inter. J. Environ. Anal. Chem. 85
• 作者: Kohji MITSUBAYASHI;et. al.
• 通讯作者: et. al.

A biochemical sniffer-chip for convenient analysis of gaseous formaldehyde from timber materials

• DOI: 10.1007/s00604-007-0799-7
• 发表时间: 2008-04
• 期刊: Microchimica Acta
• 影响因子: 5.7
• 作者: K. Mitsubayashi;G. Nishio;M. Sawai;Elito Kazawa;Hiromichi Yoshida;Takao Saito;H. Kudo;K. Otsuka-K.-Otsuk