Function of newly identified basal lamina structure fractone and regulation of neural stem cell differentiation at subventricular zone

新发现的基底层结构fractone的功能及其对室下区神经干细胞分化的调节

基本信息

批准号：
16390048
负责人：
NINOMIYA Yoshifumi
金额：
$ 9.22万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

We analyzed molecular components of the new basement membrane-like structure of capillaries in the brain, fractone. We also tried identification and separation of adult neural stem cells existing subventricular zone, and investigated mechanisms of stem cell differentiation.[1]Molecular and cellular analysis of the new basement membrane like structure fractone.We detected the basement mambrane molecules such as laminin and type IV collagen in the mouse fractone by immunohistochemistry. Especially, several laminin chains and type IV collagen chains were present in the fractone, but fibronectin was not expressed there. Moreover, the fractone was distributed around almost all of subventricular zone in the brain and spinal code but it was not present in some areas of the third and forth ventricle. Developmental studies showed that the fractone started its expression around subventricular zone at postnatal day seven, and then expression pattern was limited to lateral part of ventricle at postnatal day 14. Furthermore, we recognized the fractone structure more precisely using immunoelectron-microscopic analysis.[2]Identification and separation of neural stem cells from subventricular zoneWe tried to separate neural stem cells from adult mouse subventricular zones and allowed them to further differentiate into neurons and astrocytes in various conditions. To set-up differentiation conditions, we prepared substrates rich in matrix macromolecules.[3]Control of stem cell differentiation by extracellular matrixWe successfully culture neurospheres prepared from subventricular zone of the lateral ventricles and set-up conditions to further differentiate into neurons and astrocytes.

我们分析了大脑中毛细血管的新基底膜样结构的分子成分。我们还尝试了对成年神经干细胞的鉴定和分离现有的下室室内区域，并研究了干细胞分化的机制。[1]新地下膜的分子和细胞分析（例如结构片剂）。我们通过inminmunoyotister in Immunoyosistrys immunoyosistrys offactementemementosichemocyemations中检测了层粘胶和IV型胶原蛋白等基底mambrane Molecules。尤其是，分子中存在几个层粘连蛋白链和IV型胶原蛋白链，但在那里未表达纤连蛋白。此外，分子酮几乎分布在大脑和脊柱中的几乎所有脑室区域周围，但在第三和第三脑室的某些区域不存在。发育研究表明，分子酮在出生后第七天开始围绕腹侧区域开始表达，然后表达模式仅限于出生后第14天的脑室的侧面部分。此外，我们更精确地识别出使用免疫电体的细胞与分离的成年茎区域的分离的分离的分离。区域并允许它们在各种条件下进一步分化为神经元和星形胶质细胞。为了设置分化条件，我们制备了富含基质大分子的底物。[3]通过细胞外矩阵对干细胞分化的控制成功地培养了从侧脑室的下室和设置条件制备的神经球，以进一步分化为神经元和星形胶质细胞。

项目成果

期刊论文数量（19）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Suppression of chndrosarcoma cells by 15-deoxy-Delta 12,14-prostaglandin J2 is associated with altered expression of Bax/Bcl-xL and p21.

15-脱氧-Delta 12,14-前列腺素 J2 对软骨肉瘤细胞的抑制与 Bax/Bcl-xL 和 p21 表达的改变有关。

DOI：
发表时间：
2005
期刊：
Biochem Biophys Res Commun. 328(2)
影响因子：
0
作者：
Shen ZN;Nishida K;Doi H;Oohashi T;Hirohata S;Ozaki T;Yoshida A;Ninomiya Y;Inoue H.
通讯作者：
Inoue H.

Smooth muscle trans-membrane sarcoglycan complex in partial bladder outlet obstruction.

部分膀胱出口梗阻中的平滑肌跨膜肌聚糖复合物。