Molecular population genetic study for pantropical plants with seadrifted seeds in aspects of their species integration and speciation

泛热带植物漂流种子的物种整合和物种形成分子群体遗传研究

基本信息

批准号：
16370043
负责人：
KAJITA Tadashi
金额：
$ 6.02万
依托单位：
Chiba University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

项目摘要

1.Hibiscus tiliaceus and its allied species : CpDNA analysis revealed the followings.i)Four allied species were speciated from a pantropical plants with seadrifted seeds : Hibiscus tiliaceus.ii)Long distance seed dispersal are confirmed over the wide distribution area.iii)Distinctive genetic diversification in H.pernambucensis between both side of American Continents, and introgression from H.tiliaceus only in Atlantic populations.Microsatellite analysis revealed the followings:i)Frequent gene flow over very wide distribution range, and local diversification of population in certain areas.ii)Possible gene flow by pollen over the Isthmus of Panama.2.Ipomoea pes-caprae : AFLP analysis suggested the followings:i)Clear genetic segregation between the two subspecies (ssp.pes-caprae and ssp.brasiliensis) that are morphologically recognized.ii)Genetic diversification of population was largest between Eastern and Western part of America continents, following East Pacific, and Africa, but all of them were less than the level between the two subspecies.iii)No clear segregation of population between the Indian Oceanic and Pacific regions, that suggest gene flow via long distance seed dispersal.3.Canavalia rosea : Phylogenetic analysis of chlroplast genes suggested the followings.i)Identical haplotypes were shared among species with sea-drifted seeds.ii)A haplotype of C. rosea distributes over all the oceanic regions.4.Rhizophora mangle : Phylogenetic analysis chloroplast DNA and population studies suggested the followings.i)Distinctive genetic diversification in R. mangle between both side of American Continents.ii)R.samoensis that belongs to IWP (Indo-West Pacific) species has the same haplotype distributed over Pacific populations of R.mangle.

1.Hibiscus tiliaceus 及其近缘种：CpDNA 分析揭示了以下内容。i) 四种近缘种是从具有海漂种子的泛热带植物：Hibiscus tiliaceus 中形成的。ii) 在广泛的分布区域内证实了长距离种子传播。iii) 独特美洲大陆两侧 H.pernambucensis 的遗传多样性以及 H.tiliaceus 的基因渗入仅在大西洋种群中。微卫星分析揭示了以下内容：i）在非常广泛的分布范围内频繁的基因流动，以及某些地区种群的局部多样化。ii）巴拿马地峡花粉可能的基因流动。2.Ipomoea pes-caprae ：AFLP 分析表明以下内容：i) 两个亚种（ssp.pes-caprae 和 ssp.brasiliensis）之间存在明显的遗传分离， ii) 美洲大陆东部和西部地区种群遗传多样性最大，其次是东太平洋和非洲，但均低于两个亚种之间的水平。 iii) 各亚种之间没有明显的种群隔离印度洋和太平洋地区，表明基因通过长距离种子传播进行流动。3.Canavaliarosea：叶绿体基因的系统发育分析表明以下内容。i）相同的单倍型是具有漂海种子的物种之间共有。ii) C. Rosea 的单倍型分布在所有海洋区域。4.Rhizophora mangle：系统发育分析叶绿体 DNA 和种群研究表明以下内容。i)Rhizophora mangle 之间的独特遗传多样性美洲大陆两侧。ii)R.samoensis 属于 IWP（印度-西太平洋）物种，具有分布在太平洋种群中的相同单倍型R.曼格尔。