Study on the neural mechanisms fix insect photoperiodism: from the photoreceptor to the effecter

昆虫光周期调节神经机制研究：从光感受器到效应器

• 批准号: 16370038
• 负责人: NUMATA Hideharu
• 金额: $ 8.15万
• 依托单位: Osaka City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16370038/
• 关键词: photoperiodism; circadian clock; insect; neuron; circadian clock gene; period; RNA干渉法; クチクラ形成リズム; timeless; ホソヘリカメムシ; ルリキンバエ; 脳間部; エクジステロイド; 概日時計ニューロン; 脳側方部; 概日活動リズム; PDF; 成虫休眠; eyelet; PERIOD

The present study aims to clarify the photoreceptor, photoperiodic clock and effecter system for photoperiodism in the bean bug Riptortus pedestris and the blow fly Protophormia terraenovae.In R. pedestris, we examined the projection from the ommatidia in the central part of the compound eye, which had been shown to be important in photoperiodic photoreception, to the brain, and then identified two kinds of neurons terminating in the retrocerebral complex including corpus allatum, the endocrine effecter of the photoperiodism. Selective ablation of these neurons showed that neurons in the pars lateralis are important in the photoperiodism. Moreover, both of the two groups of neurons in the pars lateralis are involved in the photoperiodism. In P. terraenovae, we described the morphology of circadian clock neurons in the brain, and found synaptic connection between circadian clock neurons and the pars lateralis neurons; the latter have been shown to be important in photoperiodism of this … More species also. From the results of ablation experiments, we suggest the involvement of the circadian clock neurons in photoperiodism. This is the first finding in the neuron level that shows the involvement of the circadian clock neurons in photoperiodism.In R. pedestris, we identified four circadian clock genes, period, cycle, vrille and cryptochrome2. However, there were no distinctive daily fluctuations in the expression of these genes, and no difference between the photoperiodic conditions. In P. terraenovae, two .circadian clock genes, period and timeless showed similar daily fluctuation in the expression, but no photoperiodic difference between the photoperiodic conditions. It has been proposed based on the experimental result obtained in other insect species that the expression pattern of circadian clock genes plays an important role in photoperiodism. However, this is not plausible in these two species, and we have to examine other mechanisms for molecular mechanisms in photoperiodism. Less

项目成果

ルリキンバエの光周性には視髄前方基部が重要である

视髓质的前基部对于普通果蝇的光周期很重要

• 发表时间: 2004
• 作者: Yasuyama;K;沼田 英治;志賀 向子
• 通讯作者: 志賀 向子

Role of ecdysteroids in reproduction and diapause in the blow fly, Protophormia terraenovae

蜕皮类固醇在丽蝇 Protophormia terraenovae 繁殖和滞育中的作用

• 发表时间: 2005
• 作者: Tanaka;A
• 通讯作者: A

ルリキンバエのPERIOD免疫陽性細胞と脳側方部ニューロン間の神経連絡

蜥蜴蝇中 PERIOD 免疫阳性细胞与侧脑神经元之间的神经通讯

• 发表时间: 2005
• 作者: Numata;H;志賀 向子
• 通讯作者: 志賀 向子

ホソヘリカメムシの光周性に関わる脳内ニューロンの形態と機能

椿象中参与光周期现象的大脑神经元的形态和功能

• 发表时间: 2004
• 作者: Numata;H;下川 佳世
• 通讯作者: 下川 佳世

ナミニクバエの休眠誘導に見られる性差とその機構

黑蝇滞育诱导中观察到的性别差异和机制

• 发表时间: 2007
• 作者: Tanaka;A;多賀谷 純
• 通讯作者: 多賀谷 純